Cargando…
Crystal structures and kinetic studies of a laboratory evolved aldehyde reductase explain the dramatic shift of its new substrate specificity
The Fe(2+)-dependent E. coli enzyme FucO catalyzes the reversible interconversion of short-chain (S)-lactaldehyde and (S)-1,2-propanediol, using NADH and NAD(+) as cofactors, respectively. Laboratory-directed evolution experiments have been carried out previously using phenylacetaldehyde as the su...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324492/ https://www.ncbi.nlm.nih.gov/pubmed/37261425 http://dx.doi.org/10.1107/S205225252300444X |