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Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering

In clinic, controlling acute coagulation after small-diameter vessel grafts transplantation is considered a primary problem. The combination of heparin with high anticoagulant efficiency and polyurethane fiber with good compliance is a good choice for vascular materials. However, blending water-solu...

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Autores principales: Zhang, Hongmei, Zhang, Qilu, Du, Juan, Zhu, Tonghe, Chen, Dian, Liu, Feiying, Dong, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324977/
https://www.ncbi.nlm.nih.gov/pubmed/37425354
http://dx.doi.org/10.3389/fbioe.2023.1187914
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author Zhang, Hongmei
Zhang, Qilu
Du, Juan
Zhu, Tonghe
Chen, Dian
Liu, Feiying
Dong, Yang
author_facet Zhang, Hongmei
Zhang, Qilu
Du, Juan
Zhu, Tonghe
Chen, Dian
Liu, Feiying
Dong, Yang
author_sort Zhang, Hongmei
collection PubMed
description In clinic, controlling acute coagulation after small-diameter vessel grafts transplantation is considered a primary problem. The combination of heparin with high anticoagulant efficiency and polyurethane fiber with good compliance is a good choice for vascular materials. However, blending water-soluble heparin with fat-soluble poly (ester-ether-urethane) urea elastomer (PEEUU) uniformly and preparing nanofibers tubular grafts with uniform morphology is a huge challenge. In this research, we have compounded PEEUU with optimized constant concentration of heparin by homogeneous emulsion blending, then spun into the hybrid PEEUU/heparin nanofibers tubular graft (H-PHNF) for replacing rats’ abdominal aorta in situ for comprehensive performance evaluation. The in vitro results demonstrated that H-PHNF was of uniform microstructure, moderate wettability, matched mechanical properties, reliable cytocompatibility, and strongest ability to promote endothelial growth. Replacement of resected abdominal artery with the H-PHNF in rat showed that the graft was capable of homogeneous hybrid heparin and significantly promoted the stabilization of vascular smooth muscle cells (VSMCs) as well as stabilizing the blood microenvironment. This research demonstrates the H-PHNF with substantial patency, indicating their potential for vascular tissue engineering.
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spelling pubmed-103249772023-07-07 Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering Zhang, Hongmei Zhang, Qilu Du, Juan Zhu, Tonghe Chen, Dian Liu, Feiying Dong, Yang Front Bioeng Biotechnol Bioengineering and Biotechnology In clinic, controlling acute coagulation after small-diameter vessel grafts transplantation is considered a primary problem. The combination of heparin with high anticoagulant efficiency and polyurethane fiber with good compliance is a good choice for vascular materials. However, blending water-soluble heparin with fat-soluble poly (ester-ether-urethane) urea elastomer (PEEUU) uniformly and preparing nanofibers tubular grafts with uniform morphology is a huge challenge. In this research, we have compounded PEEUU with optimized constant concentration of heparin by homogeneous emulsion blending, then spun into the hybrid PEEUU/heparin nanofibers tubular graft (H-PHNF) for replacing rats’ abdominal aorta in situ for comprehensive performance evaluation. The in vitro results demonstrated that H-PHNF was of uniform microstructure, moderate wettability, matched mechanical properties, reliable cytocompatibility, and strongest ability to promote endothelial growth. Replacement of resected abdominal artery with the H-PHNF in rat showed that the graft was capable of homogeneous hybrid heparin and significantly promoted the stabilization of vascular smooth muscle cells (VSMCs) as well as stabilizing the blood microenvironment. This research demonstrates the H-PHNF with substantial patency, indicating their potential for vascular tissue engineering. Frontiers Media S.A. 2023-06-22 /pmc/articles/PMC10324977/ /pubmed/37425354 http://dx.doi.org/10.3389/fbioe.2023.1187914 Text en Copyright © 2023 Zhang, Zhang, Du, Zhu, Chen, Liu and Dong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Hongmei
Zhang, Qilu
Du, Juan
Zhu, Tonghe
Chen, Dian
Liu, Feiying
Dong, Yang
Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
title Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
title_full Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
title_fullStr Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
title_full_unstemmed Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
title_short Nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
title_sort nanofibers with homogeneous heparin distribution and protracted release profile for vascular tissue engineering
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324977/
https://www.ncbi.nlm.nih.gov/pubmed/37425354
http://dx.doi.org/10.3389/fbioe.2023.1187914
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