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Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation

The routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake...

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Autores principales: Perera, Palalle G. Tharushi, Vilagosh, Zoltan, Linklater, Denver, Nguyen, The Hong Phong, Appadoo, Dominique, Vongsvivut, Jitraporn, Tobin, Mark, Dekiwadia, Chaitali, Croft, Rodney, Ivanova, Elena P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325012/
https://www.ncbi.nlm.nih.gov/pubmed/37338043
http://dx.doi.org/10.1107/S1600577523004228
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author Perera, Palalle G. Tharushi
Vilagosh, Zoltan
Linklater, Denver
Nguyen, The Hong Phong
Appadoo, Dominique
Vongsvivut, Jitraporn
Tobin, Mark
Dekiwadia, Chaitali
Croft, Rodney
Ivanova, Elena P.
author_facet Perera, Palalle G. Tharushi
Vilagosh, Zoltan
Linklater, Denver
Nguyen, The Hong Phong
Appadoo, Dominique
Vongsvivut, Jitraporn
Tobin, Mark
Dekiwadia, Chaitali
Croft, Rodney
Ivanova, Elena P.
author_sort Perera, Palalle G. Tharushi
collection PubMed
description The routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake of nanospheres; nonetheless, cellular localization of the nanospheres remained unclear. This study utilized silica core-shell gold nanospheres (AuSi NS) of diameter 50 ± 5 nm to investigate the fate of nanospheres inside pheochromocytoma (PC 12) cells following SS THz exposure. Fluorescence microscopy was used to confirm nanosphere internalization following 10 min of SS THz exposure in the range 0.5–20 THz. Transmission electron microscopy followed by scanning transmission electron microscopy energy-dispersive spectroscopic (STEM-EDS) analysis was used to confirm the presence of AuSi NS in the cytoplasm or membrane, as single NS or in clusters (22% and 52%, respectively), with the remainder (26%) sequestered in vacuoles. Cellular uptake of NS in response to SS THz radiation could have suitable applications in a vast number of biomedical applications, regenerative medicine, vaccines, cancer therapy, gene and drug delivery.
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spelling pubmed-103250122023-07-07 Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation Perera, Palalle G. Tharushi Vilagosh, Zoltan Linklater, Denver Nguyen, The Hong Phong Appadoo, Dominique Vongsvivut, Jitraporn Tobin, Mark Dekiwadia, Chaitali Croft, Rodney Ivanova, Elena P. J Synchrotron Radiat Research Papers The routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake of nanospheres; nonetheless, cellular localization of the nanospheres remained unclear. This study utilized silica core-shell gold nanospheres (AuSi NS) of diameter 50 ± 5 nm to investigate the fate of nanospheres inside pheochromocytoma (PC 12) cells following SS THz exposure. Fluorescence microscopy was used to confirm nanosphere internalization following 10 min of SS THz exposure in the range 0.5–20 THz. Transmission electron microscopy followed by scanning transmission electron microscopy energy-dispersive spectroscopic (STEM-EDS) analysis was used to confirm the presence of AuSi NS in the cytoplasm or membrane, as single NS or in clusters (22% and 52%, respectively), with the remainder (26%) sequestered in vacuoles. Cellular uptake of NS in response to SS THz radiation could have suitable applications in a vast number of biomedical applications, regenerative medicine, vaccines, cancer therapy, gene and drug delivery. International Union of Crystallography 2023-06-20 /pmc/articles/PMC10325012/ /pubmed/37338043 http://dx.doi.org/10.1107/S1600577523004228 Text en © Palalle G. Tharushi Perera et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Perera, Palalle G. Tharushi
Vilagosh, Zoltan
Linklater, Denver
Nguyen, The Hong Phong
Appadoo, Dominique
Vongsvivut, Jitraporn
Tobin, Mark
Dekiwadia, Chaitali
Croft, Rodney
Ivanova, Elena P.
Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
title Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
title_full Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
title_fullStr Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
title_full_unstemmed Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
title_short Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
title_sort translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325012/
https://www.ncbi.nlm.nih.gov/pubmed/37338043
http://dx.doi.org/10.1107/S1600577523004228
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