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Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation
The routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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International Union of Crystallography
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325012/ https://www.ncbi.nlm.nih.gov/pubmed/37338043 http://dx.doi.org/10.1107/S1600577523004228 |
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author | Perera, Palalle G. Tharushi Vilagosh, Zoltan Linklater, Denver Nguyen, The Hong Phong Appadoo, Dominique Vongsvivut, Jitraporn Tobin, Mark Dekiwadia, Chaitali Croft, Rodney Ivanova, Elena P. |
author_facet | Perera, Palalle G. Tharushi Vilagosh, Zoltan Linklater, Denver Nguyen, The Hong Phong Appadoo, Dominique Vongsvivut, Jitraporn Tobin, Mark Dekiwadia, Chaitali Croft, Rodney Ivanova, Elena P. |
author_sort | Perera, Palalle G. Tharushi |
collection | PubMed |
description | The routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake of nanospheres; nonetheless, cellular localization of the nanospheres remained unclear. This study utilized silica core-shell gold nanospheres (AuSi NS) of diameter 50 ± 5 nm to investigate the fate of nanospheres inside pheochromocytoma (PC 12) cells following SS THz exposure. Fluorescence microscopy was used to confirm nanosphere internalization following 10 min of SS THz exposure in the range 0.5–20 THz. Transmission electron microscopy followed by scanning transmission electron microscopy energy-dispersive spectroscopic (STEM-EDS) analysis was used to confirm the presence of AuSi NS in the cytoplasm or membrane, as single NS or in clusters (22% and 52%, respectively), with the remainder (26%) sequestered in vacuoles. Cellular uptake of NS in response to SS THz radiation could have suitable applications in a vast number of biomedical applications, regenerative medicine, vaccines, cancer therapy, gene and drug delivery. |
format | Online Article Text |
id | pubmed-10325012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-103250122023-07-07 Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation Perera, Palalle G. Tharushi Vilagosh, Zoltan Linklater, Denver Nguyen, The Hong Phong Appadoo, Dominique Vongsvivut, Jitraporn Tobin, Mark Dekiwadia, Chaitali Croft, Rodney Ivanova, Elena P. J Synchrotron Radiat Research Papers The routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake of nanospheres; nonetheless, cellular localization of the nanospheres remained unclear. This study utilized silica core-shell gold nanospheres (AuSi NS) of diameter 50 ± 5 nm to investigate the fate of nanospheres inside pheochromocytoma (PC 12) cells following SS THz exposure. Fluorescence microscopy was used to confirm nanosphere internalization following 10 min of SS THz exposure in the range 0.5–20 THz. Transmission electron microscopy followed by scanning transmission electron microscopy energy-dispersive spectroscopic (STEM-EDS) analysis was used to confirm the presence of AuSi NS in the cytoplasm or membrane, as single NS or in clusters (22% and 52%, respectively), with the remainder (26%) sequestered in vacuoles. Cellular uptake of NS in response to SS THz radiation could have suitable applications in a vast number of biomedical applications, regenerative medicine, vaccines, cancer therapy, gene and drug delivery. International Union of Crystallography 2023-06-20 /pmc/articles/PMC10325012/ /pubmed/37338043 http://dx.doi.org/10.1107/S1600577523004228 Text en © Palalle G. Tharushi Perera et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Perera, Palalle G. Tharushi Vilagosh, Zoltan Linklater, Denver Nguyen, The Hong Phong Appadoo, Dominique Vongsvivut, Jitraporn Tobin, Mark Dekiwadia, Chaitali Croft, Rodney Ivanova, Elena P. Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
title | Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
title_full | Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
title_fullStr | Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
title_full_unstemmed | Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
title_short | Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
title_sort | translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325012/ https://www.ncbi.nlm.nih.gov/pubmed/37338043 http://dx.doi.org/10.1107/S1600577523004228 |
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