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Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction

A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substra...

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Autores principales: Bruns, Hendrik, Hoeppe, Hannes, Bellec, Ewen, Leake, Steven, Osterhoff, Markus, Salditt, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325018/
https://www.ncbi.nlm.nih.gov/pubmed/37233735
http://dx.doi.org/10.1107/S1600577523003685
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author Bruns, Hendrik
Hoeppe, Hannes
Bellec, Ewen
Leake, Steven
Osterhoff, Markus
Salditt, Tim
author_facet Bruns, Hendrik
Hoeppe, Hannes
Bellec, Ewen
Leake, Steven
Osterhoff, Markus
Salditt, Tim
author_sort Bruns, Hendrik
collection PubMed
description A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substrate can support several droplets at a time. Evaporation is prevented by covering the droplet by a thin film of mineral oil. In this windowless fluid which minimizes background signal, single particles can be probed and manipulated by micropipettes, which can easily be inserted and steered in the droplet. Holographic X-ray imaging is shown to be well suited to observe and monitor the pipettes, as well as the droplet surface and the particles. Aspiration and force generation are also enabled based on an application of controlled pressure differences. Experimental challenges are addressed and first results are presented, obtained at two different undulator endstations with nano-focused beams. Finally, the sample environment is discussed in view of future coherent imaging and diffraction experiments with synchrotron radiation and single X-ray free-electron laser pulses.
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spelling pubmed-103250182023-07-07 Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction Bruns, Hendrik Hoeppe, Hannes Bellec, Ewen Leake, Steven Osterhoff, Markus Salditt, Tim J Synchrotron Radiat Research Papers A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substrate can support several droplets at a time. Evaporation is prevented by covering the droplet by a thin film of mineral oil. In this windowless fluid which minimizes background signal, single particles can be probed and manipulated by micropipettes, which can easily be inserted and steered in the droplet. Holographic X-ray imaging is shown to be well suited to observe and monitor the pipettes, as well as the droplet surface and the particles. Aspiration and force generation are also enabled based on an application of controlled pressure differences. Experimental challenges are addressed and first results are presented, obtained at two different undulator endstations with nano-focused beams. Finally, the sample environment is discussed in view of future coherent imaging and diffraction experiments with synchrotron radiation and single X-ray free-electron laser pulses. International Union of Crystallography 2023-05-26 /pmc/articles/PMC10325018/ /pubmed/37233735 http://dx.doi.org/10.1107/S1600577523003685 Text en © Hendrik Bruns et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Bruns, Hendrik
Hoeppe, Hannes
Bellec, Ewen
Leake, Steven
Osterhoff, Markus
Salditt, Tim
Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
title Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
title_full Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
title_fullStr Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
title_full_unstemmed Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
title_short Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
title_sort micropipette aspiration as a tool for single-particle x-ray imaging and diffraction
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325018/
https://www.ncbi.nlm.nih.gov/pubmed/37233735
http://dx.doi.org/10.1107/S1600577523003685
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