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Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction
A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substra...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325018/ https://www.ncbi.nlm.nih.gov/pubmed/37233735 http://dx.doi.org/10.1107/S1600577523003685 |
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author | Bruns, Hendrik Hoeppe, Hannes Bellec, Ewen Leake, Steven Osterhoff, Markus Salditt, Tim |
author_facet | Bruns, Hendrik Hoeppe, Hannes Bellec, Ewen Leake, Steven Osterhoff, Markus Salditt, Tim |
author_sort | Bruns, Hendrik |
collection | PubMed |
description | A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substrate can support several droplets at a time. Evaporation is prevented by covering the droplet by a thin film of mineral oil. In this windowless fluid which minimizes background signal, single particles can be probed and manipulated by micropipettes, which can easily be inserted and steered in the droplet. Holographic X-ray imaging is shown to be well suited to observe and monitor the pipettes, as well as the droplet surface and the particles. Aspiration and force generation are also enabled based on an application of controlled pressure differences. Experimental challenges are addressed and first results are presented, obtained at two different undulator endstations with nano-focused beams. Finally, the sample environment is discussed in view of future coherent imaging and diffraction experiments with synchrotron radiation and single X-ray free-electron laser pulses. |
format | Online Article Text |
id | pubmed-10325018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-103250182023-07-07 Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction Bruns, Hendrik Hoeppe, Hannes Bellec, Ewen Leake, Steven Osterhoff, Markus Salditt, Tim J Synchrotron Radiat Research Papers A sample environment and manipulation tool is presented for single-particle X-ray experiments in an aqueous environment. The system is based on a single water droplet, positioned on a substrate that is structured by a hydrophobic and hydrophilic pattern to stabilize the droplet position. The substrate can support several droplets at a time. Evaporation is prevented by covering the droplet by a thin film of mineral oil. In this windowless fluid which minimizes background signal, single particles can be probed and manipulated by micropipettes, which can easily be inserted and steered in the droplet. Holographic X-ray imaging is shown to be well suited to observe and monitor the pipettes, as well as the droplet surface and the particles. Aspiration and force generation are also enabled based on an application of controlled pressure differences. Experimental challenges are addressed and first results are presented, obtained at two different undulator endstations with nano-focused beams. Finally, the sample environment is discussed in view of future coherent imaging and diffraction experiments with synchrotron radiation and single X-ray free-electron laser pulses. International Union of Crystallography 2023-05-26 /pmc/articles/PMC10325018/ /pubmed/37233735 http://dx.doi.org/10.1107/S1600577523003685 Text en © Hendrik Bruns et al. 2023 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Bruns, Hendrik Hoeppe, Hannes Bellec, Ewen Leake, Steven Osterhoff, Markus Salditt, Tim Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction |
title | Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction |
title_full | Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction |
title_fullStr | Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction |
title_full_unstemmed | Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction |
title_short | Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction |
title_sort | micropipette aspiration as a tool for single-particle x-ray imaging and diffraction |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10325018/ https://www.ncbi.nlm.nih.gov/pubmed/37233735 http://dx.doi.org/10.1107/S1600577523003685 |
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