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The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis

INTRODUCTION: This study aimed to understand the transcriptome characteristics of the skeletal muscle of elderly (EL) men with metabolic syndrome (MS) and to find the hub genes and insight into the molecular mechanisms of skeletal muscle in the occurrence and development of MS. METHODS: In this stud...

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Autores principales: Ge, Xing, Wang, Qingqing, Yao, Tingting, Xie, Jiafei, Zhang, Chaoran, Xu, Li-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: S. Karger AG 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326321/
https://www.ncbi.nlm.nih.gov/pubmed/37054694
http://dx.doi.org/10.1159/000530216
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author Ge, Xing
Wang, Qingqing
Yao, Tingting
Xie, Jiafei
Zhang, Chaoran
Xu, Li-Chun
author_facet Ge, Xing
Wang, Qingqing
Yao, Tingting
Xie, Jiafei
Zhang, Chaoran
Xu, Li-Chun
author_sort Ge, Xing
collection PubMed
description INTRODUCTION: This study aimed to understand the transcriptome characteristics of the skeletal muscle of elderly (EL) men with metabolic syndrome (MS) and to find the hub genes and insight into the molecular mechanisms of skeletal muscle in the occurrence and development of MS. METHODS: In this study, the limma package of R software was used to analyze the differentially expressed genes in the skeletal muscle of healthy young (YO) adult men, healthy EL men, and EL men diagnosed with MS (SX) for at least 10 years. Bioinformatics methods, such as GO enrichment analysis, KEGG enrichment analysis, and gene interaction network analysis, were used to explore the biological functions of differentially expressed genes, and weighted gene coexpression network analysis (WGCNA) was used to cluster differentially expressed genes into modules. RESULTS: Among the YO group, EL group, and SX group, 65 co-differentially expressed genes were found maybe regulated by age factor and MS factor. Those co-differentially expressed genes were enriched into 25 biological process terms and 3 KEGG pathways. Based on the WGCNA results, a total of five modules were identified. Fifteen hub genes may play an essential role in regulating the function of skeletal muscle of EL men with MS. CONCLUSIONS: 65 differentially expressed genes and 5 modules may regulate the function of skeletal muscle of EL men with MS, among which fifteen hub genes may play an essential role in the occurrence and development of MS.
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spelling pubmed-103263212023-07-08 The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis Ge, Xing Wang, Qingqing Yao, Tingting Xie, Jiafei Zhang, Chaoran Xu, Li-Chun Obes Facts Research Article INTRODUCTION: This study aimed to understand the transcriptome characteristics of the skeletal muscle of elderly (EL) men with metabolic syndrome (MS) and to find the hub genes and insight into the molecular mechanisms of skeletal muscle in the occurrence and development of MS. METHODS: In this study, the limma package of R software was used to analyze the differentially expressed genes in the skeletal muscle of healthy young (YO) adult men, healthy EL men, and EL men diagnosed with MS (SX) for at least 10 years. Bioinformatics methods, such as GO enrichment analysis, KEGG enrichment analysis, and gene interaction network analysis, were used to explore the biological functions of differentially expressed genes, and weighted gene coexpression network analysis (WGCNA) was used to cluster differentially expressed genes into modules. RESULTS: Among the YO group, EL group, and SX group, 65 co-differentially expressed genes were found maybe regulated by age factor and MS factor. Those co-differentially expressed genes were enriched into 25 biological process terms and 3 KEGG pathways. Based on the WGCNA results, a total of five modules were identified. Fifteen hub genes may play an essential role in regulating the function of skeletal muscle of EL men with MS. CONCLUSIONS: 65 differentially expressed genes and 5 modules may regulate the function of skeletal muscle of EL men with MS, among which fifteen hub genes may play an essential role in the occurrence and development of MS. S. Karger AG 2023-04-13 /pmc/articles/PMC10326321/ /pubmed/37054694 http://dx.doi.org/10.1159/000530216 Text en © 2023 The Author(s). Published by S. Karger AG, Basel https://creativecommons.org/licenses/by-nc/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC) (http://www.karger.com/Services/OpenAccessLicense). Usage and distribution for commercial purposes requires written permission.
spellingShingle Research Article
Ge, Xing
Wang, Qingqing
Yao, Tingting
Xie, Jiafei
Zhang, Chaoran
Xu, Li-Chun
The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis
title The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis
title_full The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis
title_fullStr The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis
title_full_unstemmed The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis
title_short The Skeletal Muscle Transcriptome Profile of Elderly Men with Metabolic Syndrome Based on Weighted Gene Co-Expression Network Analysis
title_sort skeletal muscle transcriptome profile of elderly men with metabolic syndrome based on weighted gene co-expression network analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326321/
https://www.ncbi.nlm.nih.gov/pubmed/37054694
http://dx.doi.org/10.1159/000530216
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