Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

Current methods to generate human primordial germ cell-like cells (hPGCLCs) from human pluripotent stem cells (hPSCs) can be inefficient, and it is challenging to generate sufficient hPGCLCs to optimize in vitro gametogenesis. We present a differentiation method that uses diluted basement membrane e...

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Detalles Bibliográficos
Autores principales: Overeem, Arend W., Chang, Yolanda W., Moustakas, Ioannis, Roelse, Celine M., Hillenius, Sanne, Helm, Talia Van Der, Schrier, Valérie F. Van Der, Gonçalves, Manuel A.F.V., Mei, Hailiang, Freund, Christian, Chuva de Sousa Lopes, Susana M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326346/
https://www.ncbi.nlm.nih.gov/pubmed/37426764
http://dx.doi.org/10.1016/j.crmeth.2023.100488

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