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DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging

Here, we present an X-ray-visible neural tracer, referred to as DiI-CT, which is based on the well-established lipophilic indocarbocyanine dye DiI, to which we conjugated two iodine atoms. The tracer is visible with microfocus computed tomography (microCT) imaging and shares the excellent fluorescen...

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Detalles Bibliográficos
Autores principales: Gerhardt, Ben, Klaue, Kristin, Eigen, Lennart, Schwarz, Jutta, Hecht, Stefan, Brecht, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326349/
https://www.ncbi.nlm.nih.gov/pubmed/37426763
http://dx.doi.org/10.1016/j.crmeth.2023.100486
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author Gerhardt, Ben
Klaue, Kristin
Eigen, Lennart
Schwarz, Jutta
Hecht, Stefan
Brecht, Michael
author_facet Gerhardt, Ben
Klaue, Kristin
Eigen, Lennart
Schwarz, Jutta
Hecht, Stefan
Brecht, Michael
author_sort Gerhardt, Ben
collection PubMed
description Here, we present an X-ray-visible neural tracer, referred to as DiI-CT, which is based on the well-established lipophilic indocarbocyanine dye DiI, to which we conjugated two iodine atoms. The tracer is visible with microfocus computed tomography (microCT) imaging and shares the excellent fluorescent tracing properties of DiI. We document the discovery potential of DiI-CT by analyzing the vibrissa follicle-sinus complex, a structure where visual access is poor and 3D tissue structure matters and reveal innervation patterns of the intact follicle in unprecedented detail. In the brain, DiI-CT tracing holds promise for verification evaluation of indirect connectivity measures, such as diffusion tensor imaging. We conclude that the bimodal dye DiI-CT opens new avenues for neuroanatomy.
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spelling pubmed-103263492023-07-08 DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging Gerhardt, Ben Klaue, Kristin Eigen, Lennart Schwarz, Jutta Hecht, Stefan Brecht, Michael Cell Rep Methods Article Here, we present an X-ray-visible neural tracer, referred to as DiI-CT, which is based on the well-established lipophilic indocarbocyanine dye DiI, to which we conjugated two iodine atoms. The tracer is visible with microfocus computed tomography (microCT) imaging and shares the excellent fluorescent tracing properties of DiI. We document the discovery potential of DiI-CT by analyzing the vibrissa follicle-sinus complex, a structure where visual access is poor and 3D tissue structure matters and reveal innervation patterns of the intact follicle in unprecedented detail. In the brain, DiI-CT tracing holds promise for verification evaluation of indirect connectivity measures, such as diffusion tensor imaging. We conclude that the bimodal dye DiI-CT opens new avenues for neuroanatomy. Elsevier 2023-05-24 /pmc/articles/PMC10326349/ /pubmed/37426763 http://dx.doi.org/10.1016/j.crmeth.2023.100486 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Gerhardt, Ben
Klaue, Kristin
Eigen, Lennart
Schwarz, Jutta
Hecht, Stefan
Brecht, Michael
DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
title DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
title_full DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
title_fullStr DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
title_full_unstemmed DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
title_short DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
title_sort dii-ct—a bimodal neural tracer for x-ray and fluorescence imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326349/
https://www.ncbi.nlm.nih.gov/pubmed/37426763
http://dx.doi.org/10.1016/j.crmeth.2023.100486
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