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DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging
Here, we present an X-ray-visible neural tracer, referred to as DiI-CT, which is based on the well-established lipophilic indocarbocyanine dye DiI, to which we conjugated two iodine atoms. The tracer is visible with microfocus computed tomography (microCT) imaging and shares the excellent fluorescen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326349/ https://www.ncbi.nlm.nih.gov/pubmed/37426763 http://dx.doi.org/10.1016/j.crmeth.2023.100486 |
_version_ | 1785069407256444928 |
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author | Gerhardt, Ben Klaue, Kristin Eigen, Lennart Schwarz, Jutta Hecht, Stefan Brecht, Michael |
author_facet | Gerhardt, Ben Klaue, Kristin Eigen, Lennart Schwarz, Jutta Hecht, Stefan Brecht, Michael |
author_sort | Gerhardt, Ben |
collection | PubMed |
description | Here, we present an X-ray-visible neural tracer, referred to as DiI-CT, which is based on the well-established lipophilic indocarbocyanine dye DiI, to which we conjugated two iodine atoms. The tracer is visible with microfocus computed tomography (microCT) imaging and shares the excellent fluorescent tracing properties of DiI. We document the discovery potential of DiI-CT by analyzing the vibrissa follicle-sinus complex, a structure where visual access is poor and 3D tissue structure matters and reveal innervation patterns of the intact follicle in unprecedented detail. In the brain, DiI-CT tracing holds promise for verification evaluation of indirect connectivity measures, such as diffusion tensor imaging. We conclude that the bimodal dye DiI-CT opens new avenues for neuroanatomy. |
format | Online Article Text |
id | pubmed-10326349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103263492023-07-08 DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging Gerhardt, Ben Klaue, Kristin Eigen, Lennart Schwarz, Jutta Hecht, Stefan Brecht, Michael Cell Rep Methods Article Here, we present an X-ray-visible neural tracer, referred to as DiI-CT, which is based on the well-established lipophilic indocarbocyanine dye DiI, to which we conjugated two iodine atoms. The tracer is visible with microfocus computed tomography (microCT) imaging and shares the excellent fluorescent tracing properties of DiI. We document the discovery potential of DiI-CT by analyzing the vibrissa follicle-sinus complex, a structure where visual access is poor and 3D tissue structure matters and reveal innervation patterns of the intact follicle in unprecedented detail. In the brain, DiI-CT tracing holds promise for verification evaluation of indirect connectivity measures, such as diffusion tensor imaging. We conclude that the bimodal dye DiI-CT opens new avenues for neuroanatomy. Elsevier 2023-05-24 /pmc/articles/PMC10326349/ /pubmed/37426763 http://dx.doi.org/10.1016/j.crmeth.2023.100486 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Gerhardt, Ben Klaue, Kristin Eigen, Lennart Schwarz, Jutta Hecht, Stefan Brecht, Michael DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging |
title | DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging |
title_full | DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging |
title_fullStr | DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging |
title_full_unstemmed | DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging |
title_short | DiI-CT—A bimodal neural tracer for X-ray and fluorescence imaging |
title_sort | dii-ct—a bimodal neural tracer for x-ray and fluorescence imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326349/ https://www.ncbi.nlm.nih.gov/pubmed/37426763 http://dx.doi.org/10.1016/j.crmeth.2023.100486 |
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