Cargando…

Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board

Telemetric electroencephalography (EEG) recording, using subdermal needle electrodes, is a minimally-invasive method to investigate mammalian neurophysiology during anesthesia. These inexpensive systems may streamline experiments examining global brain phenomena during surgical anesthesia or disease...

Descripción completa

Detalles Bibliográficos
Autores principales: Mansouri, Mohammad T., Ahmed, Meah T., Cassim, Tuan Z., Kreuzer, Matthias, Graves, Morgan C., Fenzl, Thomas, García, Paul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326441/
https://www.ncbi.nlm.nih.gov/pubmed/37424756
http://dx.doi.org/10.1016/j.mex.2023.102187
_version_ 1785069429093040128
author Mansouri, Mohammad T.
Ahmed, Meah T.
Cassim, Tuan Z.
Kreuzer, Matthias
Graves, Morgan C.
Fenzl, Thomas
García, Paul S.
author_facet Mansouri, Mohammad T.
Ahmed, Meah T.
Cassim, Tuan Z.
Kreuzer, Matthias
Graves, Morgan C.
Fenzl, Thomas
García, Paul S.
author_sort Mansouri, Mohammad T.
collection PubMed
description Telemetric electroencephalography (EEG) recording, using subdermal needle electrodes, is a minimally-invasive method to investigate mammalian neurophysiology during anesthesia. These inexpensive systems may streamline experiments examining global brain phenomena during surgical anesthesia or disease. We utilized the OpenBCI™ Cyton board with subdermal needle electrodes to extract EEG features in six C57BL/6J mice undergoing isoflurane anesthesia. Burst suppression ratio (BSR) and spectral features were compared for a verification of our method. Following an increase from 1.5% to 2.0% isoflurane, the BSR increased (Wilcoxon-signed-rank statistic; p = 0.0313). Furthermore, although the absolute EEG spectral power decreased, the relative spectral power remained comparable (Wilcoxon-Mann-Whitney U-Statistic; 95% CI exclusive AUC=0.5; p < 0.05). Compared to tethered systems, this method confers several improvements for anesthesia specific protocols: 1-Avoiding electrode implant surgical procedures, 2-Anatomical non-specificity for needle electrode placement to monitor global cortical activity representative of anesthetic state, 3-Facility to repeat recordings in the same animal, 4-User-friendly for non-experts, 5-Rapid set-up time, and 6-Lower costs. • Minimally-invasive telemetric EEG recording systems ergonomically improve tethered systems for anesthesia protocols. • Using this method, we verified that higher isoflurane concentrations resulted in an increased EEG burst suppression ratio and decreased EEG absolute spectral power, with no change in frequency distribution.
format Online
Article
Text
id pubmed-10326441
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-103264412023-07-08 Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board Mansouri, Mohammad T. Ahmed, Meah T. Cassim, Tuan Z. Kreuzer, Matthias Graves, Morgan C. Fenzl, Thomas García, Paul S. MethodsX Neuroscience Telemetric electroencephalography (EEG) recording, using subdermal needle electrodes, is a minimally-invasive method to investigate mammalian neurophysiology during anesthesia. These inexpensive systems may streamline experiments examining global brain phenomena during surgical anesthesia or disease. We utilized the OpenBCI™ Cyton board with subdermal needle electrodes to extract EEG features in six C57BL/6J mice undergoing isoflurane anesthesia. Burst suppression ratio (BSR) and spectral features were compared for a verification of our method. Following an increase from 1.5% to 2.0% isoflurane, the BSR increased (Wilcoxon-signed-rank statistic; p = 0.0313). Furthermore, although the absolute EEG spectral power decreased, the relative spectral power remained comparable (Wilcoxon-Mann-Whitney U-Statistic; 95% CI exclusive AUC=0.5; p < 0.05). Compared to tethered systems, this method confers several improvements for anesthesia specific protocols: 1-Avoiding electrode implant surgical procedures, 2-Anatomical non-specificity for needle electrode placement to monitor global cortical activity representative of anesthetic state, 3-Facility to repeat recordings in the same animal, 4-User-friendly for non-experts, 5-Rapid set-up time, and 6-Lower costs. • Minimally-invasive telemetric EEG recording systems ergonomically improve tethered systems for anesthesia protocols. • Using this method, we verified that higher isoflurane concentrations resulted in an increased EEG burst suppression ratio and decreased EEG absolute spectral power, with no change in frequency distribution. Elsevier 2023-04-15 /pmc/articles/PMC10326441/ /pubmed/37424756 http://dx.doi.org/10.1016/j.mex.2023.102187 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Neuroscience
Mansouri, Mohammad T.
Ahmed, Meah T.
Cassim, Tuan Z.
Kreuzer, Matthias
Graves, Morgan C.
Fenzl, Thomas
García, Paul S.
Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board
title Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board
title_full Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board
title_fullStr Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board
title_full_unstemmed Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board
title_short Telemetric electroencephalography recording in anesthetized mice—A novel system using minimally-invasive needle electrodes with a wireless OpenBCI™ Cyton Biosensing Board
title_sort telemetric electroencephalography recording in anesthetized mice—a novel system using minimally-invasive needle electrodes with a wireless openbci™ cyton biosensing board
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326441/
https://www.ncbi.nlm.nih.gov/pubmed/37424756
http://dx.doi.org/10.1016/j.mex.2023.102187
work_keys_str_mv AT mansourimohammadt telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard
AT ahmedmeaht telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard
AT cassimtuanz telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard
AT kreuzermatthias telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard
AT gravesmorganc telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard
AT fenzlthomas telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard
AT garciapauls telemetricelectroencephalographyrecordinginanesthetizedmiceanovelsystemusingminimallyinvasiveneedleelectrodeswithawirelessopenbcicytonbiosensingboard