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Characterizing a standardized BioPart for PVQ-specific expression in C. elegans

Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expression. P nlp-17 ::mScarlet showed bright, persistent, and spe...

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Detalles Bibliográficos
Autores principales: AlHarbi, Sarah, Frøkjær-Jensen, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326622/
https://www.ncbi.nlm.nih.gov/pubmed/37426742
http://dx.doi.org/10.17912/micropub.biology.000870
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author AlHarbi, Sarah
Frøkjær-Jensen, Christian
author_facet AlHarbi, Sarah
Frøkjær-Jensen, Christian
author_sort AlHarbi, Sarah
collection PubMed
description Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expression. P nlp-17 ::mScarlet showed bright, persistent, and specific expression in hermaphrodite and male PVQ neurons from multicopy arrays and single-copy insertions starting from the comma stage. We generated standardized P nlp-17 cloning vectors with gfp and mScarlet compatible with single-copy or array expression for PVQ-specific transgene expression or identification. To facilitate gene synthesis, we have incorporated P nlp-17 as a standard BioPart in our online transgene design tool (www.wormbuilder.org/transgenebuilder).
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spelling pubmed-103266222023-07-08 Characterizing a standardized BioPart for PVQ-specific expression in C. elegans AlHarbi, Sarah Frøkjær-Jensen, Christian MicroPubl Biol Materials and Reagents Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expression. P nlp-17 ::mScarlet showed bright, persistent, and specific expression in hermaphrodite and male PVQ neurons from multicopy arrays and single-copy insertions starting from the comma stage. We generated standardized P nlp-17 cloning vectors with gfp and mScarlet compatible with single-copy or array expression for PVQ-specific transgene expression or identification. To facilitate gene synthesis, we have incorporated P nlp-17 as a standard BioPart in our online transgene design tool (www.wormbuilder.org/transgenebuilder). Caltech Library 2023-06-22 /pmc/articles/PMC10326622/ /pubmed/37426742 http://dx.doi.org/10.17912/micropub.biology.000870 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
AlHarbi, Sarah
Frøkjær-Jensen, Christian
Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
title Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
title_full Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
title_fullStr Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
title_full_unstemmed Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
title_short Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
title_sort characterizing a standardized biopart for pvq-specific expression in c. elegans
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326622/
https://www.ncbi.nlm.nih.gov/pubmed/37426742
http://dx.doi.org/10.17912/micropub.biology.000870
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