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Characterizing a standardized BioPart for PVQ-specific expression in C. elegans
Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expression. P nlp-17 ::mScarlet showed bright, persistent, and spe...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326622/ https://www.ncbi.nlm.nih.gov/pubmed/37426742 http://dx.doi.org/10.17912/micropub.biology.000870 |
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author | AlHarbi, Sarah Frøkjær-Jensen, Christian |
author_facet | AlHarbi, Sarah Frøkjær-Jensen, Christian |
author_sort | AlHarbi, Sarah |
collection | PubMed |
description | Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expression. P nlp-17 ::mScarlet showed bright, persistent, and specific expression in hermaphrodite and male PVQ neurons from multicopy arrays and single-copy insertions starting from the comma stage. We generated standardized P nlp-17 cloning vectors with gfp and mScarlet compatible with single-copy or array expression for PVQ-specific transgene expression or identification. To facilitate gene synthesis, we have incorporated P nlp-17 as a standard BioPart in our online transgene design tool (www.wormbuilder.org/transgenebuilder). |
format | Online Article Text |
id | pubmed-10326622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-103266222023-07-08 Characterizing a standardized BioPart for PVQ-specific expression in C. elegans AlHarbi, Sarah Frøkjær-Jensen, Christian MicroPubl Biol Materials and Reagents Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expression. P nlp-17 ::mScarlet showed bright, persistent, and specific expression in hermaphrodite and male PVQ neurons from multicopy arrays and single-copy insertions starting from the comma stage. We generated standardized P nlp-17 cloning vectors with gfp and mScarlet compatible with single-copy or array expression for PVQ-specific transgene expression or identification. To facilitate gene synthesis, we have incorporated P nlp-17 as a standard BioPart in our online transgene design tool (www.wormbuilder.org/transgenebuilder). Caltech Library 2023-06-22 /pmc/articles/PMC10326622/ /pubmed/37426742 http://dx.doi.org/10.17912/micropub.biology.000870 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Materials and Reagents AlHarbi, Sarah Frøkjær-Jensen, Christian Characterizing a standardized BioPart for PVQ-specific expression in C. elegans |
title |
Characterizing a standardized BioPart for PVQ-specific expression in
C. elegans
|
title_full |
Characterizing a standardized BioPart for PVQ-specific expression in
C. elegans
|
title_fullStr |
Characterizing a standardized BioPart for PVQ-specific expression in
C. elegans
|
title_full_unstemmed |
Characterizing a standardized BioPart for PVQ-specific expression in
C. elegans
|
title_short |
Characterizing a standardized BioPart for PVQ-specific expression in
C. elegans
|
title_sort | characterizing a standardized biopart for pvq-specific expression in
c. elegans |
topic | Materials and Reagents |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326622/ https://www.ncbi.nlm.nih.gov/pubmed/37426742 http://dx.doi.org/10.17912/micropub.biology.000870 |
work_keys_str_mv | AT alharbisarah characterizingastandardizedbiopartforpvqspecificexpressionincelegans AT frøkjærjensenchristian characterizingastandardizedbiopartforpvqspecificexpressionincelegans |