Cargando…

Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents

Trypanosoma theileri is a worldwide distributed haemoparasite that has been reported throughout the American continent in various species, including bovines, buffaloes and bats. In bovines, high incidence of T. theileri can be harmful when associated with other infections or under stress situations....

Descripción completa

Detalles Bibliográficos
Autores principales: Chávez-Larrea, María Augusta, Cholota-Iza, Cristina, Cueva-Villavicencio, Jorge, Yugcha-Díaz, Michelle, Ron-Román, Jorge Washington, Rodríguez-Cabezas, Andrea, Saegerman, Claude, Reyna-Bello, Armando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326842/
https://www.ncbi.nlm.nih.gov/pubmed/37426080
http://dx.doi.org/10.3389/fvets.2023.1153069
_version_ 1785069507091365888
author Chávez-Larrea, María Augusta
Cholota-Iza, Cristina
Cueva-Villavicencio, Jorge
Yugcha-Díaz, Michelle
Ron-Román, Jorge Washington
Rodríguez-Cabezas, Andrea
Saegerman, Claude
Reyna-Bello, Armando
author_facet Chávez-Larrea, María Augusta
Cholota-Iza, Cristina
Cueva-Villavicencio, Jorge
Yugcha-Díaz, Michelle
Ron-Román, Jorge Washington
Rodríguez-Cabezas, Andrea
Saegerman, Claude
Reyna-Bello, Armando
author_sort Chávez-Larrea, María Augusta
collection PubMed
description Trypanosoma theileri is a worldwide distributed haemoparasite that has been reported throughout the American continent in various species, including bovines, buffaloes and bats. In bovines, high incidence of T. theileri can be harmful when associated with other infections or under stress situations. There is little information on this hemoflagellate in Ecuador, which prompted the study and molecular identification of the trypanosomes collected in two slaughtering centers. Between February and April 2021, a total of 218 samples of bovine blood were collected in abattoirs located in the Andean region of Quito (n = 83) and in the coastal region, in Santo Domingo (n = 135). Quito public Slaughterhouse is the biggest in Ecuador, and for that, they receive animals from all country; on the other hand, Santo Domingo's Slaughterhouse is a small one where mainly females from the region are sacrificed and some males. The samples were evaluated using two molecular tests, the PCR cathepsin L-like (CatL) specific for T. theileri and for the positive samples, a Nested PCR that targets the ITS of the 18S gene. The corresponding PCR products were sequenced, analyzed by BLAST/NCBI and the sequences were used to build a concatenated phylogenetic tree, using the MEGA XI software. Overall, 34 out of the 218 samples, (15.6%) were positive to T. theileri by PCR CatL, resulting from 20/83 (24.1%) positives from the Quito abattoir and 14/135 (10.4%) from the Santo Domingo slaughterhouse. These prevalence rates were found to be significantly different (p = 0.006). According to the phylogenetic tree based on the CatL and ITS concatenated sequences (n = 13), the two novel Equatorial T. theileri isolates, ThI (n = 7) and ThII (n = 6) are closely related and associated to the IC, IB and IIB genotypes, present in Brazil, Venezuela and Colombia. Thirty-one out of the thirty-four T. theileri-positive bovines were co-infected with other haemotropic pathogens, Anaplasma marginale Babesia spp and T. vivax. This coinfection could be responsible for additional pathologies and harmful effects on the affected cattle. This study presents the molecular identification and genotypification of T. theileri isolated from cattle in Ecuador through the analysis of CAtL and ITS sequences, and the high frequency of coinfection of this hemoflagellate with other blood haemotropic organisms.
format Online
Article
Text
id pubmed-10326842
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-103268422023-07-08 Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents Chávez-Larrea, María Augusta Cholota-Iza, Cristina Cueva-Villavicencio, Jorge Yugcha-Díaz, Michelle Ron-Román, Jorge Washington Rodríguez-Cabezas, Andrea Saegerman, Claude Reyna-Bello, Armando Front Vet Sci Veterinary Science Trypanosoma theileri is a worldwide distributed haemoparasite that has been reported throughout the American continent in various species, including bovines, buffaloes and bats. In bovines, high incidence of T. theileri can be harmful when associated with other infections or under stress situations. There is little information on this hemoflagellate in Ecuador, which prompted the study and molecular identification of the trypanosomes collected in two slaughtering centers. Between February and April 2021, a total of 218 samples of bovine blood were collected in abattoirs located in the Andean region of Quito (n = 83) and in the coastal region, in Santo Domingo (n = 135). Quito public Slaughterhouse is the biggest in Ecuador, and for that, they receive animals from all country; on the other hand, Santo Domingo's Slaughterhouse is a small one where mainly females from the region are sacrificed and some males. The samples were evaluated using two molecular tests, the PCR cathepsin L-like (CatL) specific for T. theileri and for the positive samples, a Nested PCR that targets the ITS of the 18S gene. The corresponding PCR products were sequenced, analyzed by BLAST/NCBI and the sequences were used to build a concatenated phylogenetic tree, using the MEGA XI software. Overall, 34 out of the 218 samples, (15.6%) were positive to T. theileri by PCR CatL, resulting from 20/83 (24.1%) positives from the Quito abattoir and 14/135 (10.4%) from the Santo Domingo slaughterhouse. These prevalence rates were found to be significantly different (p = 0.006). According to the phylogenetic tree based on the CatL and ITS concatenated sequences (n = 13), the two novel Equatorial T. theileri isolates, ThI (n = 7) and ThII (n = 6) are closely related and associated to the IC, IB and IIB genotypes, present in Brazil, Venezuela and Colombia. Thirty-one out of the thirty-four T. theileri-positive bovines were co-infected with other haemotropic pathogens, Anaplasma marginale Babesia spp and T. vivax. This coinfection could be responsible for additional pathologies and harmful effects on the affected cattle. This study presents the molecular identification and genotypification of T. theileri isolated from cattle in Ecuador through the analysis of CAtL and ITS sequences, and the high frequency of coinfection of this hemoflagellate with other blood haemotropic organisms. Frontiers Media S.A. 2023-06-23 /pmc/articles/PMC10326842/ /pubmed/37426080 http://dx.doi.org/10.3389/fvets.2023.1153069 Text en Copyright © 2023 Chávez-Larrea, Cholota-Iza, Cueva-Villavicencio, Yugcha-Díaz, Ron-Román, Rodríguez-Cabezas, Saegerman and Reyna-Bello. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Chávez-Larrea, María Augusta
Cholota-Iza, Cristina
Cueva-Villavicencio, Jorge
Yugcha-Díaz, Michelle
Ron-Román, Jorge Washington
Rodríguez-Cabezas, Andrea
Saegerman, Claude
Reyna-Bello, Armando
Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents
title Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents
title_full Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents
title_fullStr Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents
title_full_unstemmed Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents
title_short Molecular identification of Trypanosoma theileri (Laveran, 1902) in cattle from two slaughterhouses in Ecuador and its relation with other haemotropic agents
title_sort molecular identification of trypanosoma theileri (laveran, 1902) in cattle from two slaughterhouses in ecuador and its relation with other haemotropic agents
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326842/
https://www.ncbi.nlm.nih.gov/pubmed/37426080
http://dx.doi.org/10.3389/fvets.2023.1153069
work_keys_str_mv AT chavezlarreamariaaugusta molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT cholotaizacristina molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT cuevavillavicenciojorge molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT yugchadiazmichelle molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT ronromanjorgewashington molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT rodriguezcabezasandrea molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT saegermanclaude molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents
AT reynabelloarmando molecularidentificationoftrypanosomatheilerilaveran1902incattlefromtwoslaughterhousesinecuadoranditsrelationwithotherhaemotropicagents