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Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy

Tumor is one of the biggest threats to human health. Though tumor therapy has been dramatically advanced by the progress of technology and research in recent decades, it is still far from expectations. Thus, it is of great significance to explore the mechanisms of tumor growth, metastasis, and resis...

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Autores principales: Li, Min, Sun, Jin, Shi, Guohai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326906/
https://www.ncbi.nlm.nih.gov/pubmed/37427373
http://dx.doi.org/10.3389/fcell.2023.1220376
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author Li, Min
Sun, Jin
Shi, Guohai
author_facet Li, Min
Sun, Jin
Shi, Guohai
author_sort Li, Min
collection PubMed
description Tumor is one of the biggest threats to human health. Though tumor therapy has been dramatically advanced by the progress of technology and research in recent decades, it is still far from expectations. Thus, it is of great significance to explore the mechanisms of tumor growth, metastasis, and resistance. Screen based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein (Cas) 9 gene editing technology are powerful tools for exploring the abovementioned facets. This review summarizes the recent screen performed in cancer cells and immune cells in the tumor microenvironment. The screens in cancer cells mainly focus on exploring the mechanisms underlying cancer cells’ growth, metastasis, and how cancer cells escape from the FDA approved drugs or immunotherapy. And the studies in tumor-associated immune cells are primarily aimed at identifying signaling pathways that can enhance the anti-tumor function of cytotoxic T lymphocytes (CTLs), CAR-T cells, and macrophages. Moreover, we discuss the limitations, merits of the CRISPR screen, and further its future application in tumor studies. Importantly, recent advances in high throughput tumor related CRISPR screen have deeply contributed to new concepts and mechanisms underlying tumor development, tumor drug resistance, and tumor immune therapy, all of which will eventually potentiate the clinical therapy for tumor patients.
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spelling pubmed-103269062023-07-08 Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy Li, Min Sun, Jin Shi, Guohai Front Cell Dev Biol Cell and Developmental Biology Tumor is one of the biggest threats to human health. Though tumor therapy has been dramatically advanced by the progress of technology and research in recent decades, it is still far from expectations. Thus, it is of great significance to explore the mechanisms of tumor growth, metastasis, and resistance. Screen based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein (Cas) 9 gene editing technology are powerful tools for exploring the abovementioned facets. This review summarizes the recent screen performed in cancer cells and immune cells in the tumor microenvironment. The screens in cancer cells mainly focus on exploring the mechanisms underlying cancer cells’ growth, metastasis, and how cancer cells escape from the FDA approved drugs or immunotherapy. And the studies in tumor-associated immune cells are primarily aimed at identifying signaling pathways that can enhance the anti-tumor function of cytotoxic T lymphocytes (CTLs), CAR-T cells, and macrophages. Moreover, we discuss the limitations, merits of the CRISPR screen, and further its future application in tumor studies. Importantly, recent advances in high throughput tumor related CRISPR screen have deeply contributed to new concepts and mechanisms underlying tumor development, tumor drug resistance, and tumor immune therapy, all of which will eventually potentiate the clinical therapy for tumor patients. Frontiers Media S.A. 2023-06-23 /pmc/articles/PMC10326906/ /pubmed/37427373 http://dx.doi.org/10.3389/fcell.2023.1220376 Text en Copyright © 2023 Li, Sun and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Li, Min
Sun, Jin
Shi, Guohai
Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
title Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
title_full Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
title_fullStr Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
title_full_unstemmed Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
title_short Application of CRISPR screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
title_sort application of crispr screen in mechanistic studies of tumor development, tumor drug resistance, and tumor immunotherapy
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326906/
https://www.ncbi.nlm.nih.gov/pubmed/37427373
http://dx.doi.org/10.3389/fcell.2023.1220376
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