Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry

[Image: see text] The detection of a single entity (molecule, cell, particle, etc.) was always a challenging subject. Here we demonstrate the detection of single Ag nanoparticles (NPs) using subatmospheric pressure laser desorption/ionization mass spectrometry (LDI MS). The sample preparation, measu...

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Autores principales: Žalud, Michal, Prysiazhnyi, Vadym, Bednařík, Antonín, Preisler, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326916/
https://www.ncbi.nlm.nih.gov/pubmed/37307240
http://dx.doi.org/10.1021/jasms.3c00137
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author Žalud, Michal
Prysiazhnyi, Vadym
Bednařík, Antonín
Preisler, Jan
author_facet Žalud, Michal
Prysiazhnyi, Vadym
Bednařík, Antonín
Preisler, Jan
author_sort Žalud, Michal
collection PubMed
description [Image: see text] The detection of a single entity (molecule, cell, particle, etc.) was always a challenging subject. Here we demonstrate the detection of single Ag nanoparticles (NPs) using subatmospheric pressure laser desorption/ionization mass spectrometry (LDI MS). The sample preparation, measurement conditions, generated ions, and limiting experimental factors are discussed here. We detected from 84 to 95% of the deposited 80 nm Ag NPs. The presented LDI MS platform is an alternative to laser ablation inductively coupled plasma mass spectrometry for imaging distribution of individual NPs across the sample surface and has a great potential for multiplexed mapping of low-abundance biomarkers in tissues.
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spelling pubmed-103269162023-07-08 Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry Žalud, Michal Prysiazhnyi, Vadym Bednařík, Antonín Preisler, Jan J Am Soc Mass Spectrom [Image: see text] The detection of a single entity (molecule, cell, particle, etc.) was always a challenging subject. Here we demonstrate the detection of single Ag nanoparticles (NPs) using subatmospheric pressure laser desorption/ionization mass spectrometry (LDI MS). The sample preparation, measurement conditions, generated ions, and limiting experimental factors are discussed here. We detected from 84 to 95% of the deposited 80 nm Ag NPs. The presented LDI MS platform is an alternative to laser ablation inductively coupled plasma mass spectrometry for imaging distribution of individual NPs across the sample surface and has a great potential for multiplexed mapping of low-abundance biomarkers in tissues. American Chemical Society 2023-06-12 /pmc/articles/PMC10326916/ /pubmed/37307240 http://dx.doi.org/10.1021/jasms.3c00137 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Žalud, Michal
Prysiazhnyi, Vadym
Bednařík, Antonín
Preisler, Jan
Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
title Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
title_full Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
title_fullStr Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
title_full_unstemmed Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
title_short Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
title_sort detection of single ag nanoparticles using laser desorption/ionization mass spectrometry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326916/
https://www.ncbi.nlm.nih.gov/pubmed/37307240
http://dx.doi.org/10.1021/jasms.3c00137
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