Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces

OBJECTIVE: Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection sensitivity using a mock community and a melamine-coated t...

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Detalles Bibliográficos
Autores principales: Shiwa, Yuh, Baba, Tomoya, Sierra, Maria A., Kim, JangKeun, Mason, Christopher E., Suzuki, Haruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326927/
https://www.ncbi.nlm.nih.gov/pubmed/37420286
http://dx.doi.org/10.1186/s13104-023-06417-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326927/
https://www.ncbi.nlm.nih.gov/pubmed/37420286
http://dx.doi.org/10.1186/s13104-023-06417-9

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