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Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces

OBJECTIVE: Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection sensitivity using a mock community and a melamine-coated t...

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Detalles Bibliográficos
Autores principales:	Shiwa, Yuh, Baba, Tomoya, Sierra, Maria A., Kim, JangKeun, Mason, Christopher E., Suzuki, Haruo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2023
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10326927/ https://www.ncbi.nlm.nih.gov/pubmed/37420286 http://dx.doi.org/10.1186/s13104-023-06417-9

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