Cargando…
Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
In pancreatic islet beta cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. Beta-cell microtubule network has a complex architecture and is non-directional, which provide insulin granules at the cell periphery for rapid...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327020/ https://www.ncbi.nlm.nih.gov/pubmed/37425827 http://dx.doi.org/10.1101/2023.06.25.546468 |
_version_ | 1785069544534966272 |
---|---|
author | Bracey, Kai M. Noguchi, Pi’illani Edwards, Courtney Cario, Alisa Gu, Guoqiang Kaverina, Irina |
author_facet | Bracey, Kai M. Noguchi, Pi’illani Edwards, Courtney Cario, Alisa Gu, Guoqiang Kaverina, Irina |
author_sort | Bracey, Kai M. |
collection | PubMed |
description | In pancreatic islet beta cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. Beta-cell microtubule network has a complex architecture and is non-directional, which provide insulin granules at the cell periphery for rapid secretion response, yet to avoid over-secretion and subsequent hypoglycemia. We have previously characterized a peripheral sub-membrane microtubule array, which is critical for withdrawal of excessive insulin granules from the secretion sites. Microtubules in beta cells originate at the Golgi in the cell interior, and how the peripheral array is formed is unknown. Using real-time imaging and photo-kinetics approaches in clonal mouse pancreatic beta cells MIN6, we now demonstrate that kinesin KIF5B, a motor protein with a capacity to transport microtubules as cargos, slides existing microtubules to the cell periphery and aligns them to each other along the plasma membrane. Moreover, like many physiological beta-cell features, microtubule sliding is facilitated by a high glucose stimulus. These new data, together with our previous report that in high glucose sub-membrane MT array is destabilized to allow for robust secretion, indicate that MT sliding is another integral part of glucose-triggered microtubule remodeling, likely replacing destabilized peripheral microtubules to prevent their loss over time and beta-cell malfunction. |
format | Online Article Text |
id | pubmed-10327020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-103270202023-07-08 Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells Bracey, Kai M. Noguchi, Pi’illani Edwards, Courtney Cario, Alisa Gu, Guoqiang Kaverina, Irina bioRxiv Article In pancreatic islet beta cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. Beta-cell microtubule network has a complex architecture and is non-directional, which provide insulin granules at the cell periphery for rapid secretion response, yet to avoid over-secretion and subsequent hypoglycemia. We have previously characterized a peripheral sub-membrane microtubule array, which is critical for withdrawal of excessive insulin granules from the secretion sites. Microtubules in beta cells originate at the Golgi in the cell interior, and how the peripheral array is formed is unknown. Using real-time imaging and photo-kinetics approaches in clonal mouse pancreatic beta cells MIN6, we now demonstrate that kinesin KIF5B, a motor protein with a capacity to transport microtubules as cargos, slides existing microtubules to the cell periphery and aligns them to each other along the plasma membrane. Moreover, like many physiological beta-cell features, microtubule sliding is facilitated by a high glucose stimulus. These new data, together with our previous report that in high glucose sub-membrane MT array is destabilized to allow for robust secretion, indicate that MT sliding is another integral part of glucose-triggered microtubule remodeling, likely replacing destabilized peripheral microtubules to prevent their loss over time and beta-cell malfunction. Cold Spring Harbor Laboratory 2023-06-26 /pmc/articles/PMC10327020/ /pubmed/37425827 http://dx.doi.org/10.1101/2023.06.25.546468 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Bracey, Kai M. Noguchi, Pi’illani Edwards, Courtney Cario, Alisa Gu, Guoqiang Kaverina, Irina Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
title | Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
title_full | Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
title_fullStr | Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
title_full_unstemmed | Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
title_short | Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
title_sort | glucose-stimulated kif5b-driven microtubule sliding organizes microtubule networks in pancreatic beta cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327020/ https://www.ncbi.nlm.nih.gov/pubmed/37425827 http://dx.doi.org/10.1101/2023.06.25.546468 |
work_keys_str_mv | AT braceykaim glucosestimulatedkif5bdrivenmicrotubuleslidingorganizesmicrotubulenetworksinpancreaticbetacells AT noguchipiillani glucosestimulatedkif5bdrivenmicrotubuleslidingorganizesmicrotubulenetworksinpancreaticbetacells AT edwardscourtney glucosestimulatedkif5bdrivenmicrotubuleslidingorganizesmicrotubulenetworksinpancreaticbetacells AT carioalisa glucosestimulatedkif5bdrivenmicrotubuleslidingorganizesmicrotubulenetworksinpancreaticbetacells AT guguoqiang glucosestimulatedkif5bdrivenmicrotubuleslidingorganizesmicrotubulenetworksinpancreaticbetacells AT kaverinairina glucosestimulatedkif5bdrivenmicrotubuleslidingorganizesmicrotubulenetworksinpancreaticbetacells |