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Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells

In pancreatic islet beta cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. Beta-cell microtubule network has a complex architecture and is non-directional, which provide insulin granules at the cell periphery for rapid...

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Autores principales: Bracey, Kai M., Noguchi, Pi’illani, Edwards, Courtney, Cario, Alisa, Gu, Guoqiang, Kaverina, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327020/
https://www.ncbi.nlm.nih.gov/pubmed/37425827
http://dx.doi.org/10.1101/2023.06.25.546468
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author Bracey, Kai M.
Noguchi, Pi’illani
Edwards, Courtney
Cario, Alisa
Gu, Guoqiang
Kaverina, Irina
author_facet Bracey, Kai M.
Noguchi, Pi’illani
Edwards, Courtney
Cario, Alisa
Gu, Guoqiang
Kaverina, Irina
author_sort Bracey, Kai M.
collection PubMed
description In pancreatic islet beta cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. Beta-cell microtubule network has a complex architecture and is non-directional, which provide insulin granules at the cell periphery for rapid secretion response, yet to avoid over-secretion and subsequent hypoglycemia. We have previously characterized a peripheral sub-membrane microtubule array, which is critical for withdrawal of excessive insulin granules from the secretion sites. Microtubules in beta cells originate at the Golgi in the cell interior, and how the peripheral array is formed is unknown. Using real-time imaging and photo-kinetics approaches in clonal mouse pancreatic beta cells MIN6, we now demonstrate that kinesin KIF5B, a motor protein with a capacity to transport microtubules as cargos, slides existing microtubules to the cell periphery and aligns them to each other along the plasma membrane. Moreover, like many physiological beta-cell features, microtubule sliding is facilitated by a high glucose stimulus. These new data, together with our previous report that in high glucose sub-membrane MT array is destabilized to allow for robust secretion, indicate that MT sliding is another integral part of glucose-triggered microtubule remodeling, likely replacing destabilized peripheral microtubules to prevent their loss over time and beta-cell malfunction.
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spelling pubmed-103270202023-07-08 Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells Bracey, Kai M. Noguchi, Pi’illani Edwards, Courtney Cario, Alisa Gu, Guoqiang Kaverina, Irina bioRxiv Article In pancreatic islet beta cells, molecular motors use cytoskeletal polymers microtubules as tracks for intracellular transport of insulin secretory granules. Beta-cell microtubule network has a complex architecture and is non-directional, which provide insulin granules at the cell periphery for rapid secretion response, yet to avoid over-secretion and subsequent hypoglycemia. We have previously characterized a peripheral sub-membrane microtubule array, which is critical for withdrawal of excessive insulin granules from the secretion sites. Microtubules in beta cells originate at the Golgi in the cell interior, and how the peripheral array is formed is unknown. Using real-time imaging and photo-kinetics approaches in clonal mouse pancreatic beta cells MIN6, we now demonstrate that kinesin KIF5B, a motor protein with a capacity to transport microtubules as cargos, slides existing microtubules to the cell periphery and aligns them to each other along the plasma membrane. Moreover, like many physiological beta-cell features, microtubule sliding is facilitated by a high glucose stimulus. These new data, together with our previous report that in high glucose sub-membrane MT array is destabilized to allow for robust secretion, indicate that MT sliding is another integral part of glucose-triggered microtubule remodeling, likely replacing destabilized peripheral microtubules to prevent their loss over time and beta-cell malfunction. Cold Spring Harbor Laboratory 2023-06-26 /pmc/articles/PMC10327020/ /pubmed/37425827 http://dx.doi.org/10.1101/2023.06.25.546468 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Bracey, Kai M.
Noguchi, Pi’illani
Edwards, Courtney
Cario, Alisa
Gu, Guoqiang
Kaverina, Irina
Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
title Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
title_full Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
title_fullStr Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
title_full_unstemmed Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
title_short Glucose-stimulated KIF5B-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
title_sort glucose-stimulated kif5b-driven microtubule sliding organizes microtubule networks in pancreatic beta cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327020/
https://www.ncbi.nlm.nih.gov/pubmed/37425827
http://dx.doi.org/10.1101/2023.06.25.546468
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