Synapsin E-domain is essential for α-synuclein function

The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows...

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Autores principales: Stavsky, Alexandra, Parra-Rivas, Leonardo A., Tal, Shani, Madhivanan, Kayalvizhi, Roy, Subhojit, Gitler, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327093/
https://www.ncbi.nlm.nih.gov/pubmed/37425805
http://dx.doi.org/10.1101/2023.06.24.546170
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author Stavsky, Alexandra
Parra-Rivas, Leonardo A.
Tal, Shani
Madhivanan, Kayalvizhi
Roy, Subhojit
Gitler, Daniel
author_facet Stavsky, Alexandra
Parra-Rivas, Leonardo A.
Tal, Shani
Madhivanan, Kayalvizhi
Roy, Subhojit
Gitler, Daniel
author_sort Stavsky, Alexandra
collection PubMed
description The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at the synapse. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters.
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spelling pubmed-103270932023-07-08 Synapsin E-domain is essential for α-synuclein function Stavsky, Alexandra Parra-Rivas, Leonardo A. Tal, Shani Madhivanan, Kayalvizhi Roy, Subhojit Gitler, Daniel bioRxiv Article The cytosolic proteins synucleins and synapsins are thought to play cooperative roles in regulating synaptic vesicle (SV) recycling, but mechanistic insight is lacking. Here we identify the synapsin E-domain as an essential functional binding-partner of α-synuclein (α-syn). Synapsin E-domain allows α-syn functionality, binds to α-syn, and is necessary and sufficient for enabling effects of α-syn at the synapse. Together with previous studies implicating the E-domain in clustering SVs, our experiments advocate a cooperative role for these two proteins in maintaining physiologic SV clusters. Cold Spring Harbor Laboratory 2023-06-26 /pmc/articles/PMC10327093/ /pubmed/37425805 http://dx.doi.org/10.1101/2023.06.24.546170 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Stavsky, Alexandra
Parra-Rivas, Leonardo A.
Tal, Shani
Madhivanan, Kayalvizhi
Roy, Subhojit
Gitler, Daniel
Synapsin E-domain is essential for α-synuclein function
title Synapsin E-domain is essential for α-synuclein function
title_full Synapsin E-domain is essential for α-synuclein function
title_fullStr Synapsin E-domain is essential for α-synuclein function
title_full_unstemmed Synapsin E-domain is essential for α-synuclein function
title_short Synapsin E-domain is essential for α-synuclein function
title_sort synapsin e-domain is essential for α-synuclein function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327093/
https://www.ncbi.nlm.nih.gov/pubmed/37425805
http://dx.doi.org/10.1101/2023.06.24.546170
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