FruitFire: a luciferase based on a fruit fly metabolic enzyme

Firefly luciferase is homologous to fatty acyl-CoA synthetases from insects that are not bioluminescent. Here, we determined the crystal structure of the fruit fly fatty acyl-CoA synthetase CG6178 to 2.5 Å. Based on this structure, we mutated a steric protrusion in the active site to create the arti...

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Detalles Bibliográficos
Autores principales: Adams, Spencer T., Zephyr, Jacqueto, Bohn, Markus F., Schiffer, Celia A., Miller, Stephen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327219/
https://www.ncbi.nlm.nih.gov/pubmed/37425765
http://dx.doi.org/10.1101/2023.06.30.547126

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327219/
https://www.ncbi.nlm.nih.gov/pubmed/37425765
http://dx.doi.org/10.1101/2023.06.30.547126

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