Cargando…
Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis
Disulfidptosis is a newly discovered mode of cell death induced by disulfide stress. However, the prognostic value of disulfidptosis-related genes (DRGs) in renal cell carcinoma (RCC) remains to be further elucidated. In this study, consistent cluster analysis was used to classify 571 RCC samples in...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327482/ https://www.ncbi.nlm.nih.gov/pubmed/37426643 http://dx.doi.org/10.3389/fimmu.2023.1205250 |
_version_ | 1785069636412243968 |
---|---|
author | Xu, Kai Zhang, Ye Yan, Zhiwei Wang, Yuchan Li, Yanze Qiu, Qiangmin Du, Yang Chen, Zhiyuan Liu, Xiuheng |
author_facet | Xu, Kai Zhang, Ye Yan, Zhiwei Wang, Yuchan Li, Yanze Qiu, Qiangmin Du, Yang Chen, Zhiyuan Liu, Xiuheng |
author_sort | Xu, Kai |
collection | PubMed |
description | Disulfidptosis is a newly discovered mode of cell death induced by disulfide stress. However, the prognostic value of disulfidptosis-related genes (DRGs) in renal cell carcinoma (RCC) remains to be further elucidated. In this study, consistent cluster analysis was used to classify 571 RCC samples into three DRG-related subtypes based on changes in DRGs expression. Through univariate regression analysis and LASSO-Cox regression analysis of differentially expressed genes (DEGs) among three subtypes, we constructed and validated a DRG risk score to predict the prognosis of patients with RCC, while also identifying three gene subtypes. Analysis of DRG risk score, clinical characteristics, tumor microenvironment (TME), somatic cell mutations, and immunotherapy sensitivity revealed significant correlations between them. A series of studies have shown that MSH3 can be a potential biomarker of RCC, and its low expression is associated with poor prognosis in patients with RCC. Last but not least, overexpression of MSH3 promotes cell death in two RCC cell lines under glucose starvation conditions, indicating that MSH3 is a key gene in the process of cell disulfidptosis. In summary, we identify potential mechanism of RCC progression through DRGs -related tumor microenvironment remodeling. In addition, this study has successfully established a new disulfidptosis-related genes prediction model and discovered a key gene MSH3. They may be new prognostic biomarkers for RCC patients, provide new insights for the treatment of RCC patients, and may inspire new methods for the diagnosis and treatment of RCC patients. |
format | Online Article Text |
id | pubmed-10327482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103274822023-07-08 Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis Xu, Kai Zhang, Ye Yan, Zhiwei Wang, Yuchan Li, Yanze Qiu, Qiangmin Du, Yang Chen, Zhiyuan Liu, Xiuheng Front Immunol Immunology Disulfidptosis is a newly discovered mode of cell death induced by disulfide stress. However, the prognostic value of disulfidptosis-related genes (DRGs) in renal cell carcinoma (RCC) remains to be further elucidated. In this study, consistent cluster analysis was used to classify 571 RCC samples into three DRG-related subtypes based on changes in DRGs expression. Through univariate regression analysis and LASSO-Cox regression analysis of differentially expressed genes (DEGs) among three subtypes, we constructed and validated a DRG risk score to predict the prognosis of patients with RCC, while also identifying three gene subtypes. Analysis of DRG risk score, clinical characteristics, tumor microenvironment (TME), somatic cell mutations, and immunotherapy sensitivity revealed significant correlations between them. A series of studies have shown that MSH3 can be a potential biomarker of RCC, and its low expression is associated with poor prognosis in patients with RCC. Last but not least, overexpression of MSH3 promotes cell death in two RCC cell lines under glucose starvation conditions, indicating that MSH3 is a key gene in the process of cell disulfidptosis. In summary, we identify potential mechanism of RCC progression through DRGs -related tumor microenvironment remodeling. In addition, this study has successfully established a new disulfidptosis-related genes prediction model and discovered a key gene MSH3. They may be new prognostic biomarkers for RCC patients, provide new insights for the treatment of RCC patients, and may inspire new methods for the diagnosis and treatment of RCC patients. Frontiers Media S.A. 2023-06-23 /pmc/articles/PMC10327482/ /pubmed/37426643 http://dx.doi.org/10.3389/fimmu.2023.1205250 Text en Copyright © 2023 Xu, Zhang, Yan, Wang, Li, Qiu, Du, Chen and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Xu, Kai Zhang, Ye Yan, Zhiwei Wang, Yuchan Li, Yanze Qiu, Qiangmin Du, Yang Chen, Zhiyuan Liu, Xiuheng Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis |
title | Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis |
title_full | Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis |
title_fullStr | Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis |
title_full_unstemmed | Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis |
title_short | Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which MSH3 is a key gene during disulfidptosis |
title_sort | identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of drg prognostic prediction model in rcc, in which msh3 is a key gene during disulfidptosis |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327482/ https://www.ncbi.nlm.nih.gov/pubmed/37426643 http://dx.doi.org/10.3389/fimmu.2023.1205250 |
work_keys_str_mv | AT xukai identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT zhangye identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT yanzhiwei identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT wangyuchan identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT liyanze identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT qiuqiangmin identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT duyang identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT chenzhiyuan identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis AT liuxiuheng identificationofdisulfidptosisrelatedsubtypescharacterizationoftumormicroenvironmentinfiltrationanddevelopmentofdrgprognosticpredictionmodelinrccinwhichmsh3isakeygeneduringdisulfidptosis |