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Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions
We report that preexisting (old) and newly synthesized (new) histones H3 and H4 are asymmetrically partitioned during the division of Drosophila intestinal stem cells (ISCs). Furthermore, the inheritance patterns of old and new H3 and H4 in postmitotic cell pairs correlate with distinct expression p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328082/ https://www.ncbi.nlm.nih.gov/pubmed/37255015 http://dx.doi.org/10.15252/embr.202256404 |
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author | Zion, Emily H Ringwalt, Daniel Rinaldi, Kristina Kahney, Elizabeth W Li, Yingying Chen, Xin |
author_facet | Zion, Emily H Ringwalt, Daniel Rinaldi, Kristina Kahney, Elizabeth W Li, Yingying Chen, Xin |
author_sort | Zion, Emily H |
collection | PubMed |
description | We report that preexisting (old) and newly synthesized (new) histones H3 and H4 are asymmetrically partitioned during the division of Drosophila intestinal stem cells (ISCs). Furthermore, the inheritance patterns of old and new H3 and H4 in postmitotic cell pairs correlate with distinct expression patterns of Delta, an important cell fate gene. To understand the biological significance of this phenomenon, we expressed a mutant H3T3A to compromise asymmetric histone inheritance. Under this condition, we observe an increase in Delta‐symmetric cell pairs and overpopulated ISC‐like, Delta‐positive cells. Single‐cell RNA‐seq assays further indicate that H3T3A expression compromises ISC differentiation. Together, our results indicate that asymmetric histone inheritance potentially contributes to establishing distinct cell identities in a somatic stem cell lineage, consistent with previous findings in Drosophila male germline stem cells. |
format | Online Article Text |
id | pubmed-10328082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103280822023-07-08 Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions Zion, Emily H Ringwalt, Daniel Rinaldi, Kristina Kahney, Elizabeth W Li, Yingying Chen, Xin EMBO Rep Reports We report that preexisting (old) and newly synthesized (new) histones H3 and H4 are asymmetrically partitioned during the division of Drosophila intestinal stem cells (ISCs). Furthermore, the inheritance patterns of old and new H3 and H4 in postmitotic cell pairs correlate with distinct expression patterns of Delta, an important cell fate gene. To understand the biological significance of this phenomenon, we expressed a mutant H3T3A to compromise asymmetric histone inheritance. Under this condition, we observe an increase in Delta‐symmetric cell pairs and overpopulated ISC‐like, Delta‐positive cells. Single‐cell RNA‐seq assays further indicate that H3T3A expression compromises ISC differentiation. Together, our results indicate that asymmetric histone inheritance potentially contributes to establishing distinct cell identities in a somatic stem cell lineage, consistent with previous findings in Drosophila male germline stem cells. John Wiley and Sons Inc. 2023-05-31 /pmc/articles/PMC10328082/ /pubmed/37255015 http://dx.doi.org/10.15252/embr.202256404 Text en © 2023 The Authors. Published under the terms of the CC BY 4.0 license. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Reports Zion, Emily H Ringwalt, Daniel Rinaldi, Kristina Kahney, Elizabeth W Li, Yingying Chen, Xin Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
title | Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
title_full | Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
title_fullStr | Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
title_full_unstemmed | Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
title_short | Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions |
title_sort | old and newly synthesized histones are asymmetrically distributed in drosophila intestinal stem cell divisions |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328082/ https://www.ncbi.nlm.nih.gov/pubmed/37255015 http://dx.doi.org/10.15252/embr.202256404 |
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