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Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats

INTRODUCTION: There are genuine concerns that long-term use of anti-retroviral drugs may be associated with reproductive complications in females. This study aimed to ascertain the effect of highly active anti-retroviral drugs on the ovarian reserve and reproductive potential of female Wistar rats a...

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Autores principales: Ohihoin, Aigbe Gregory, Ohihoin, Esther Ngozi, Ujomu, Ifeoma, Bakare, Airat, Olanrewaju, Oladeji, Okafor, Arinze, Ojetunde, Mercy, Ayoola, Joy Boluwatife, Aina, Oluwagbemiga, Ajibaye, Olusola, Taylor-Robinson, Simon D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328220/
https://www.ncbi.nlm.nih.gov/pubmed/37425074
http://dx.doi.org/10.2147/JEP.S398343
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author Ohihoin, Aigbe Gregory
Ohihoin, Esther Ngozi
Ujomu, Ifeoma
Bakare, Airat
Olanrewaju, Oladeji
Okafor, Arinze
Ojetunde, Mercy
Ayoola, Joy Boluwatife
Aina, Oluwagbemiga
Ajibaye, Olusola
Taylor-Robinson, Simon D
author_facet Ohihoin, Aigbe Gregory
Ohihoin, Esther Ngozi
Ujomu, Ifeoma
Bakare, Airat
Olanrewaju, Oladeji
Okafor, Arinze
Ojetunde, Mercy
Ayoola, Joy Boluwatife
Aina, Oluwagbemiga
Ajibaye, Olusola
Taylor-Robinson, Simon D
author_sort Ohihoin, Aigbe Gregory
collection PubMed
description INTRODUCTION: There are genuine concerns that long-term use of anti-retroviral drugs may be associated with reproductive complications in females. This study aimed to ascertain the effect of highly active anti-retroviral drugs on the ovarian reserve and reproductive potential of female Wistar rats and by extension to HIV-positive human females. METHODS: A total of 25 female Wistar rats, weighing between 140g and 162g, were randomly allotted into non-intervention and intervention groups, receiving the anti-retroviral drugs, Efavirenz (EFV), Tenofovir Disoproxil Fumarate (TDF), Lamivudine (3TC), and a fixed-dose combination (FDC). The dosage was administered orally at 8 am daily for 4 weeks. Serum concentrations of anti-Mullerian hormone (AMH), follicle-stimulating hormone (FSH), luteinising hormone (LH), and estradiol were measured using standard biochemical techniques (ELISA). Follicular counts were made on fixed ovarian tissue from the sacrificed rats. RESULTS: The mean AMH level for the control group and the EFV, TDF, 3TC, and FDC groups were 11.20, 6.75, 7.30, 8.27, and 6.60 pmol/L, respectively. The EFV and FDC groups had the lowest AMH, compared to the other groups, but there was no statistically significant difference in AMH across the groups. The mean count of antral follicles was significantly lower in the group that received EFV when compared to the other groups. The corpus luteal count was significantly higher in the control group than in the intervention groups. CONCLUSION: The study demonstrated a disruption in the reproductive hormones of female Wistar rats receiving anti-retroviral regimens containing EFV. Clinical studies are required to determine if these changes are seen in women receiving EFV-based treatment, as this may compromise reproductive function and predispose them to early menopause.
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spelling pubmed-103282202023-07-08 Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats Ohihoin, Aigbe Gregory Ohihoin, Esther Ngozi Ujomu, Ifeoma Bakare, Airat Olanrewaju, Oladeji Okafor, Arinze Ojetunde, Mercy Ayoola, Joy Boluwatife Aina, Oluwagbemiga Ajibaye, Olusola Taylor-Robinson, Simon D J Exp Pharmacol Original Research INTRODUCTION: There are genuine concerns that long-term use of anti-retroviral drugs may be associated with reproductive complications in females. This study aimed to ascertain the effect of highly active anti-retroviral drugs on the ovarian reserve and reproductive potential of female Wistar rats and by extension to HIV-positive human females. METHODS: A total of 25 female Wistar rats, weighing between 140g and 162g, were randomly allotted into non-intervention and intervention groups, receiving the anti-retroviral drugs, Efavirenz (EFV), Tenofovir Disoproxil Fumarate (TDF), Lamivudine (3TC), and a fixed-dose combination (FDC). The dosage was administered orally at 8 am daily for 4 weeks. Serum concentrations of anti-Mullerian hormone (AMH), follicle-stimulating hormone (FSH), luteinising hormone (LH), and estradiol were measured using standard biochemical techniques (ELISA). Follicular counts were made on fixed ovarian tissue from the sacrificed rats. RESULTS: The mean AMH level for the control group and the EFV, TDF, 3TC, and FDC groups were 11.20, 6.75, 7.30, 8.27, and 6.60 pmol/L, respectively. The EFV and FDC groups had the lowest AMH, compared to the other groups, but there was no statistically significant difference in AMH across the groups. The mean count of antral follicles was significantly lower in the group that received EFV when compared to the other groups. The corpus luteal count was significantly higher in the control group than in the intervention groups. CONCLUSION: The study demonstrated a disruption in the reproductive hormones of female Wistar rats receiving anti-retroviral regimens containing EFV. Clinical studies are required to determine if these changes are seen in women receiving EFV-based treatment, as this may compromise reproductive function and predispose them to early menopause. Dove 2023-07-03 /pmc/articles/PMC10328220/ /pubmed/37425074 http://dx.doi.org/10.2147/JEP.S398343 Text en © 2023 Ohihoin et al. https://creativecommons.org/licenses/by/4.0/This work is published by Dove Medical Press Limited, and licensed under a Creative Commons Attribution License. The full terms of the License are available at http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Research
Ohihoin, Aigbe Gregory
Ohihoin, Esther Ngozi
Ujomu, Ifeoma
Bakare, Airat
Olanrewaju, Oladeji
Okafor, Arinze
Ojetunde, Mercy
Ayoola, Joy Boluwatife
Aina, Oluwagbemiga
Ajibaye, Olusola
Taylor-Robinson, Simon D
Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats
title Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats
title_full Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats
title_fullStr Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats
title_full_unstemmed Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats
title_short Contemporary Anti-Retroviral Drugs (ARVDs) Disrupt Follicular Development in Female Wistar Rats
title_sort contemporary anti-retroviral drugs (arvds) disrupt follicular development in female wistar rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328220/
https://www.ncbi.nlm.nih.gov/pubmed/37425074
http://dx.doi.org/10.2147/JEP.S398343
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