Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format

Plate-based proteomic sample preparation offers a solution to the large sample throughput demands in the biotechnology field where hundreds or thousands of engineered microbes are constructed for testing is routine. Meanwhile, sample preparation methods that work efficiently on broader microbial gro...

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Detalles Bibliográficos
Autores principales: Chen, Yan, Gin, Jennifer W., Wang, Ying, de Raad, Markus, Tan, Stephen, Hillson, Nathan J., Northen, Trent R., Adams, Paul D., Petzold, Christopher J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Lab Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328223/
https://www.ncbi.nlm.nih.gov/pubmed/37418444
http://dx.doi.org/10.1371/journal.pone.0288102

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