Cargando…
Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia
Murine typhus (MT), an infection caused by the gram-negative bacteria Rickettsia typhi (R. typhi), is a significant cause of acute febrile illness (AFI) in Southeast Asia but is rarely reported in Indonesia. The current study aimed to describe the clinical characteristics of MT cases in Bandung, Wes...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328256/ https://www.ncbi.nlm.nih.gov/pubmed/37418452 http://dx.doi.org/10.1371/journal.pone.0283135 |
_version_ | 1785069756019113984 |
---|---|
author | Riswari, Silvita Fitri Prodjosoewojo, Susantina Mony, Siti Rasnawati Megantara, Imam Iskandar, Shelly Mayasari, Wulan Heryaman, Henhen de Mast, Quirijn van der Ven, Andre Kosasih, Herman Alisjahbana, Bachti |
author_facet | Riswari, Silvita Fitri Prodjosoewojo, Susantina Mony, Siti Rasnawati Megantara, Imam Iskandar, Shelly Mayasari, Wulan Heryaman, Henhen de Mast, Quirijn van der Ven, Andre Kosasih, Herman Alisjahbana, Bachti |
author_sort | Riswari, Silvita Fitri |
collection | PubMed |
description | Murine typhus (MT), an infection caused by the gram-negative bacteria Rickettsia typhi (R. typhi), is a significant cause of acute febrile illness (AFI) in Southeast Asia but is rarely reported in Indonesia. The current study aimed to describe the clinical characteristics of MT cases in Bandung, West Java. Non-confirmed AFI cases (n = 176) from a prospective cohort study of whom paired serum samples (acute (T1), midterm (T2), or convalescent (T3)) were available were screened using MT serology. IgG against R. typhi was detected in the T2 or T3 samples using an in-house ELISA. Positive IgG samples were further screened for the presence of IgM. If both IgM and IgG were positive, the endpoint titer of T1, T2, or T3 was determined. In cases with a fourfold increase in titer, real-time PCR of T1 samples was performed to detect R. typhi DNA. In total, 71/176 (40.3%) patients tested positive for IgG antibody, and 26 AFI cases were confirmed as MT (23 cases by PCR, 3 cases by fourfold titer increased IgG or IgM titer). The most common clinical symptoms in the confirmed cases were headache (80%), arthralgia (73%), malaise (69%), and myalgia (54%). In these cases, the presumptive clinical diagnoses were typhoid fever (43.2%), dengue (38.5%), and leptospirosis (19.2%). MT was not considered in any of the patients, and no patients received doxycycline. These findings confirmed that MT is an important cause of AFI in Indonesia. MT should be included in the differential diagnosis of AFI, and empirical treatment with doxycycline should be considered. |
format | Online Article Text |
id | pubmed-10328256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-103282562023-07-08 Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia Riswari, Silvita Fitri Prodjosoewojo, Susantina Mony, Siti Rasnawati Megantara, Imam Iskandar, Shelly Mayasari, Wulan Heryaman, Henhen de Mast, Quirijn van der Ven, Andre Kosasih, Herman Alisjahbana, Bachti PLoS One Research Article Murine typhus (MT), an infection caused by the gram-negative bacteria Rickettsia typhi (R. typhi), is a significant cause of acute febrile illness (AFI) in Southeast Asia but is rarely reported in Indonesia. The current study aimed to describe the clinical characteristics of MT cases in Bandung, West Java. Non-confirmed AFI cases (n = 176) from a prospective cohort study of whom paired serum samples (acute (T1), midterm (T2), or convalescent (T3)) were available were screened using MT serology. IgG against R. typhi was detected in the T2 or T3 samples using an in-house ELISA. Positive IgG samples were further screened for the presence of IgM. If both IgM and IgG were positive, the endpoint titer of T1, T2, or T3 was determined. In cases with a fourfold increase in titer, real-time PCR of T1 samples was performed to detect R. typhi DNA. In total, 71/176 (40.3%) patients tested positive for IgG antibody, and 26 AFI cases were confirmed as MT (23 cases by PCR, 3 cases by fourfold titer increased IgG or IgM titer). The most common clinical symptoms in the confirmed cases were headache (80%), arthralgia (73%), malaise (69%), and myalgia (54%). In these cases, the presumptive clinical diagnoses were typhoid fever (43.2%), dengue (38.5%), and leptospirosis (19.2%). MT was not considered in any of the patients, and no patients received doxycycline. These findings confirmed that MT is an important cause of AFI in Indonesia. MT should be included in the differential diagnosis of AFI, and empirical treatment with doxycycline should be considered. Public Library of Science 2023-07-07 /pmc/articles/PMC10328256/ /pubmed/37418452 http://dx.doi.org/10.1371/journal.pone.0283135 Text en © 2023 Riswari et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Riswari, Silvita Fitri Prodjosoewojo, Susantina Mony, Siti Rasnawati Megantara, Imam Iskandar, Shelly Mayasari, Wulan Heryaman, Henhen de Mast, Quirijn van der Ven, Andre Kosasih, Herman Alisjahbana, Bachti Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia |
title | Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia |
title_full | Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia |
title_fullStr | Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia |
title_full_unstemmed | Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia |
title_short | Murine typhus is a common cause of acute febrile illness in Bandung, Indonesia |
title_sort | murine typhus is a common cause of acute febrile illness in bandung, indonesia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328256/ https://www.ncbi.nlm.nih.gov/pubmed/37418452 http://dx.doi.org/10.1371/journal.pone.0283135 |
work_keys_str_mv | AT riswarisilvitafitri murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT prodjosoewojosusantina murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT monysitirasnawati murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT megantaraimam murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT iskandarshelly murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT mayasariwulan murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT heryamanhenhen murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT demastquirijn murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT vandervenandre murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT kosasihherman murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia AT alisjahbanabachti murinetyphusisacommoncauseofacutefebrileillnessinbandungindonesia |