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Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke

[Image: see text] Exosomes derived from human bone marrow mesenchymal stem cells (MSC-Exo) are characterized by easy expansion and storage, low risk of tumor formation, low immunogenicity, and anti-inflammatory effects. The therapeutic effects of MSC-Exo on ischemic stroke have been widely explored....

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Autores principales: Liu, Chang, Yang, Tian-Hui, Li, Hong-Dan, Li, Gong-Zhe, Liang, Jia, Wang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328279/
https://www.ncbi.nlm.nih.gov/pubmed/37056144
http://dx.doi.org/10.4103/1673-5374.369114
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author Liu, Chang
Yang, Tian-Hui
Li, Hong-Dan
Li, Gong-Zhe
Liang, Jia
Wang, Peng
author_facet Liu, Chang
Yang, Tian-Hui
Li, Hong-Dan
Li, Gong-Zhe
Liang, Jia
Wang, Peng
author_sort Liu, Chang
collection PubMed
description [Image: see text] Exosomes derived from human bone marrow mesenchymal stem cells (MSC-Exo) are characterized by easy expansion and storage, low risk of tumor formation, low immunogenicity, and anti-inflammatory effects. The therapeutic effects of MSC-Exo on ischemic stroke have been widely explored. However, the underlying mechanism remains unclear. In this study, we established a mouse model of ischemic brain injury induced by occlusion of the middle cerebral artery using the thread bolt method and injected MSC-Exo into the tail vein. We found that administration of MSC-Exo reduced the volume of cerebral infarction in the ischemic brain injury mouse model, increased the levels of interleukin-33 (IL-33) and suppression of tumorigenicity 2 receptor (ST2) in the penumbra of cerebral infarction, and improved neurological function. In vitro results showed that astrocyte-conditioned medium of cells deprived of both oxygen and glucose, to simulate ischemia conditions, combined with MSC-Exo increased the survival rate of primary cortical neurons. However, after transfection by IL-33 siRNA or ST2 siRNA, the survival rate of primary cortical neurons was markedly decreased. These results indicated that MSC-Exo inhibited neuronal death induced by oxygen and glucose deprivation through the IL-33/ST2 signaling pathway in astrocytes. These findings suggest that MSC-Exo may reduce ischemia-induced brain injury through regulating the IL-33/ST2 signaling pathway. Therefore, MSC-Exo may be a potential therapeutic method for ischemic stroke.
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spelling pubmed-103282792023-07-08 Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke Liu, Chang Yang, Tian-Hui Li, Hong-Dan Li, Gong-Zhe Liang, Jia Wang, Peng Neural Regen Res Research Article [Image: see text] Exosomes derived from human bone marrow mesenchymal stem cells (MSC-Exo) are characterized by easy expansion and storage, low risk of tumor formation, low immunogenicity, and anti-inflammatory effects. The therapeutic effects of MSC-Exo on ischemic stroke have been widely explored. However, the underlying mechanism remains unclear. In this study, we established a mouse model of ischemic brain injury induced by occlusion of the middle cerebral artery using the thread bolt method and injected MSC-Exo into the tail vein. We found that administration of MSC-Exo reduced the volume of cerebral infarction in the ischemic brain injury mouse model, increased the levels of interleukin-33 (IL-33) and suppression of tumorigenicity 2 receptor (ST2) in the penumbra of cerebral infarction, and improved neurological function. In vitro results showed that astrocyte-conditioned medium of cells deprived of both oxygen and glucose, to simulate ischemia conditions, combined with MSC-Exo increased the survival rate of primary cortical neurons. However, after transfection by IL-33 siRNA or ST2 siRNA, the survival rate of primary cortical neurons was markedly decreased. These results indicated that MSC-Exo inhibited neuronal death induced by oxygen and glucose deprivation through the IL-33/ST2 signaling pathway in astrocytes. These findings suggest that MSC-Exo may reduce ischemia-induced brain injury through regulating the IL-33/ST2 signaling pathway. Therefore, MSC-Exo may be a potential therapeutic method for ischemic stroke. Wolters Kluwer - Medknow 2023-03-03 /pmc/articles/PMC10328279/ /pubmed/37056144 http://dx.doi.org/10.4103/1673-5374.369114 Text en Copyright: © 2023 Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons AttributionNonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Liu, Chang
Yang, Tian-Hui
Li, Hong-Dan
Li, Gong-Zhe
Liang, Jia
Wang, Peng
Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
title Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
title_full Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
title_fullStr Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
title_full_unstemmed Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
title_short Exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
title_sort exosomes from bone marrow mesenchymal stem cells are a potential treatment for ischemic stroke
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328279/
https://www.ncbi.nlm.nih.gov/pubmed/37056144
http://dx.doi.org/10.4103/1673-5374.369114
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