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Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires

Cancer cell–derived extracellular vesicles (EVs) have unique protein profiles, making them promising targets as disease biomarkers. High-grade serous ovarian carcinoma (HGSOC) is the deadly subtype of epithelial ovarian cancer, and we aimed to identify HGSOC-specific membrane proteins. Small EVs (sE...

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Autores principales: Yokoi, Akira, Ukai, Mayu, Yasui, Takao, Inokuma, Yasuhide, Hyeon-Deuk, Kim, Matsuzaki, Juntaro, Yoshida, Kosuke, Kitagawa, Masami, Chattrairat, Kunanon, Iida, Mikiko, Shimada, Taisuke, Manabe, Yumehiro, Chang, I-Ya, Asano-Inami, Eri, Koya, Yoshihiro, Nawa, Akihiro, Nakamura, Kae, Kiyono, Tohru, Kato, Tomoyasu, Hirakawa, Akihiko, Yoshioka, Yusuke, Ochiya, Takahiro, Hasegawa, Takeshi, Baba, Yoshinobu, Yamamoto, Yusuke, Kajiyama, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328412/
https://www.ncbi.nlm.nih.gov/pubmed/37418532
http://dx.doi.org/10.1126/sciadv.ade6958
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author Yokoi, Akira
Ukai, Mayu
Yasui, Takao
Inokuma, Yasuhide
Hyeon-Deuk, Kim
Matsuzaki, Juntaro
Yoshida, Kosuke
Kitagawa, Masami
Chattrairat, Kunanon
Iida, Mikiko
Shimada, Taisuke
Manabe, Yumehiro
Chang, I-Ya
Asano-Inami, Eri
Koya, Yoshihiro
Nawa, Akihiro
Nakamura, Kae
Kiyono, Tohru
Kato, Tomoyasu
Hirakawa, Akihiko
Yoshioka, Yusuke
Ochiya, Takahiro
Hasegawa, Takeshi
Baba, Yoshinobu
Yamamoto, Yusuke
Kajiyama, Hiroaki
author_facet Yokoi, Akira
Ukai, Mayu
Yasui, Takao
Inokuma, Yasuhide
Hyeon-Deuk, Kim
Matsuzaki, Juntaro
Yoshida, Kosuke
Kitagawa, Masami
Chattrairat, Kunanon
Iida, Mikiko
Shimada, Taisuke
Manabe, Yumehiro
Chang, I-Ya
Asano-Inami, Eri
Koya, Yoshihiro
Nawa, Akihiro
Nakamura, Kae
Kiyono, Tohru
Kato, Tomoyasu
Hirakawa, Akihiko
Yoshioka, Yusuke
Ochiya, Takahiro
Hasegawa, Takeshi
Baba, Yoshinobu
Yamamoto, Yusuke
Kajiyama, Hiroaki
author_sort Yokoi, Akira
collection PubMed
description Cancer cell–derived extracellular vesicles (EVs) have unique protein profiles, making them promising targets as disease biomarkers. High-grade serous ovarian carcinoma (HGSOC) is the deadly subtype of epithelial ovarian cancer, and we aimed to identify HGSOC-specific membrane proteins. Small EVs (sEVs) and medium/large EVs (m/lEVs) from cell lines or patient serum and ascites were analyzed by LC-MS/MS, revealing that both EV subtypes had unique proteomic characteristics. Multivalidation steps identified FRα, Claudin-3, and TACSTD2 as HGSOC-specific sEV proteins, but m/lEV-associated candidates were not identified. In addition, for using a simple-to-use microfluidic device for EV isolation, polyketone-coated nanowires (pNWs) were developed, which efficiently purify sEVs from biofluids. Multiplexed array assays of sEVs isolated by pNW showed specific detectability in cancer patients and predicted clinical status. In summary, the HGSOC-specific marker detection by pNW are a promising platform as clinical biomarkers, and these insights provide detailed proteomic aspects of diverse EVs in HGSOC patients.
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spelling pubmed-103284122023-07-08 Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires Yokoi, Akira Ukai, Mayu Yasui, Takao Inokuma, Yasuhide Hyeon-Deuk, Kim Matsuzaki, Juntaro Yoshida, Kosuke Kitagawa, Masami Chattrairat, Kunanon Iida, Mikiko Shimada, Taisuke Manabe, Yumehiro Chang, I-Ya Asano-Inami, Eri Koya, Yoshihiro Nawa, Akihiro Nakamura, Kae Kiyono, Tohru Kato, Tomoyasu Hirakawa, Akihiko Yoshioka, Yusuke Ochiya, Takahiro Hasegawa, Takeshi Baba, Yoshinobu Yamamoto, Yusuke Kajiyama, Hiroaki Sci Adv Biomedicine and Life Sciences Cancer cell–derived extracellular vesicles (EVs) have unique protein profiles, making them promising targets as disease biomarkers. High-grade serous ovarian carcinoma (HGSOC) is the deadly subtype of epithelial ovarian cancer, and we aimed to identify HGSOC-specific membrane proteins. Small EVs (sEVs) and medium/large EVs (m/lEVs) from cell lines or patient serum and ascites were analyzed by LC-MS/MS, revealing that both EV subtypes had unique proteomic characteristics. Multivalidation steps identified FRα, Claudin-3, and TACSTD2 as HGSOC-specific sEV proteins, but m/lEV-associated candidates were not identified. In addition, for using a simple-to-use microfluidic device for EV isolation, polyketone-coated nanowires (pNWs) were developed, which efficiently purify sEVs from biofluids. Multiplexed array assays of sEVs isolated by pNW showed specific detectability in cancer patients and predicted clinical status. In summary, the HGSOC-specific marker detection by pNW are a promising platform as clinical biomarkers, and these insights provide detailed proteomic aspects of diverse EVs in HGSOC patients. American Association for the Advancement of Science 2023-07-07 /pmc/articles/PMC10328412/ /pubmed/37418532 http://dx.doi.org/10.1126/sciadv.ade6958 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Yokoi, Akira
Ukai, Mayu
Yasui, Takao
Inokuma, Yasuhide
Hyeon-Deuk, Kim
Matsuzaki, Juntaro
Yoshida, Kosuke
Kitagawa, Masami
Chattrairat, Kunanon
Iida, Mikiko
Shimada, Taisuke
Manabe, Yumehiro
Chang, I-Ya
Asano-Inami, Eri
Koya, Yoshihiro
Nawa, Akihiro
Nakamura, Kae
Kiyono, Tohru
Kato, Tomoyasu
Hirakawa, Akihiko
Yoshioka, Yusuke
Ochiya, Takahiro
Hasegawa, Takeshi
Baba, Yoshinobu
Yamamoto, Yusuke
Kajiyama, Hiroaki
Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
title Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
title_full Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
title_fullStr Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
title_full_unstemmed Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
title_short Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
title_sort identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328412/
https://www.ncbi.nlm.nih.gov/pubmed/37418532
http://dx.doi.org/10.1126/sciadv.ade6958
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