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Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry

This work describes a novel automated and rapid method for bottom-up proteomics combining protein isolation with a micro-immobilised enzyme reactor (IMER). Crosslinking chemistry based on 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling was exploited to immobili...

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Detalles Bibliográficos
Autores principales:	Duong, Karen, Maleknia, Simin, Clases, David, Minett, Andrew, Padula, Matthew P., Doble, Philip A., Gonzalez de Vega, Raquel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2022
Materias:	Paper in Forefront
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328895/ https://www.ncbi.nlm.nih.gov/pubmed/36369591 http://dx.doi.org/10.1007/s00216-022-04381-0

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