Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq

Adipose tissue plays a central role in age-related diseases. While RNAseq protocols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we present a protocol to analyze the transcriptional changes that o...

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Detalles Bibliográficos
Autores principales: De Cauwer, Aurore, Pichot, Angélique, Molitor, Anne, Stemmelen, Tristan, Carapito, Raphael, Bahram, Seiamak, Georgel, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328976/
https://www.ncbi.nlm.nih.gov/pubmed/37393615
http://dx.doi.org/10.1016/j.xpro.2023.102397
Descripción
Sumario:Adipose tissue plays a central role in age-related diseases. While RNAseq protocols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we present a protocol to analyze the transcriptional changes that occur in adipose tissue during normal and accelerated aging in mouse models. We describe steps for genotyping, diet control, euthanasia, and dissection. We then detail RNA purification and genome-wide data generation and analysis. For complete details on the use and execution of this protocol, please refer to De Cauwer et al. (2022) iScience. Sep 16;25(10):105149.

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