Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq

Adipose tissue plays a central role in age-related diseases. While RNAseq protocols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we present a protocol to analyze the transcriptional changes that o...

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Autores principales: De Cauwer, Aurore, Pichot, Angélique, Molitor, Anne, Stemmelen, Tristan, Carapito, Raphael, Bahram, Seiamak, Georgel, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328976/
https://www.ncbi.nlm.nih.gov/pubmed/37393615
http://dx.doi.org/10.1016/j.xpro.2023.102397
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author De Cauwer, Aurore
Pichot, Angélique
Molitor, Anne
Stemmelen, Tristan
Carapito, Raphael
Bahram, Seiamak
Georgel, Philippe
author_facet De Cauwer, Aurore
Pichot, Angélique
Molitor, Anne
Stemmelen, Tristan
Carapito, Raphael
Bahram, Seiamak
Georgel, Philippe
author_sort De Cauwer, Aurore
collection PubMed
description Adipose tissue plays a central role in age-related diseases. While RNAseq protocols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we present a protocol to analyze the transcriptional changes that occur in adipose tissue during normal and accelerated aging in mouse models. We describe steps for genotyping, diet control, euthanasia, and dissection. We then detail RNA purification and genome-wide data generation and analysis. For complete details on the use and execution of this protocol, please refer to De Cauwer et al. (2022) iScience. Sep 16;25(10):105149.
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spelling pubmed-103289762023-07-09 Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq De Cauwer, Aurore Pichot, Angélique Molitor, Anne Stemmelen, Tristan Carapito, Raphael Bahram, Seiamak Georgel, Philippe STAR Protoc Protocol Adipose tissue plays a central role in age-related diseases. While RNAseq protocols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we present a protocol to analyze the transcriptional changes that occur in adipose tissue during normal and accelerated aging in mouse models. We describe steps for genotyping, diet control, euthanasia, and dissection. We then detail RNA purification and genome-wide data generation and analysis. For complete details on the use and execution of this protocol, please refer to De Cauwer et al. (2022) iScience. Sep 16;25(10):105149. Elsevier 2023-07-01 /pmc/articles/PMC10328976/ /pubmed/37393615 http://dx.doi.org/10.1016/j.xpro.2023.102397 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
De Cauwer, Aurore
Pichot, Angélique
Molitor, Anne
Stemmelen, Tristan
Carapito, Raphael
Bahram, Seiamak
Georgel, Philippe
Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq
title Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq
title_full Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq
title_fullStr Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq
title_full_unstemmed Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq
title_short Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq
title_sort measuring the transcriptome-wide effects of aging on murine adipocytes using rnaseq
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10328976/
https://www.ncbi.nlm.nih.gov/pubmed/37393615
http://dx.doi.org/10.1016/j.xpro.2023.102397
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