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Ceramide releases exosomes with a specific miRNA signature for cell differentiation

Exosomes are well established effectors of cell–cell communication. Their role on maturation of embryonic cells located in hippocampus, seat of memory, is unknown. Here we show that ceramide facilitates release of exosomes from HN9.10e cells extending information for cell differentiation to neighbor...

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Autores principales: Fiorani, Federico, Domenis, Rossana, Dalla, Emiliano, Cataldi, Samuela, Conte, Carmela, Mandarano, Martina, Sidoni, Angelo, Cifù, Adriana, Beccari, Tommaso, Mirarchi, Alessandra, Arcuri, Cataldo, Curcio, Francesco, Albi, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329022/
https://www.ncbi.nlm.nih.gov/pubmed/37419964
http://dx.doi.org/10.1038/s41598-023-38011-1
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author Fiorani, Federico
Domenis, Rossana
Dalla, Emiliano
Cataldi, Samuela
Conte, Carmela
Mandarano, Martina
Sidoni, Angelo
Cifù, Adriana
Beccari, Tommaso
Mirarchi, Alessandra
Arcuri, Cataldo
Curcio, Francesco
Albi, Elisabetta
author_facet Fiorani, Federico
Domenis, Rossana
Dalla, Emiliano
Cataldi, Samuela
Conte, Carmela
Mandarano, Martina
Sidoni, Angelo
Cifù, Adriana
Beccari, Tommaso
Mirarchi, Alessandra
Arcuri, Cataldo
Curcio, Francesco
Albi, Elisabetta
author_sort Fiorani, Federico
collection PubMed
description Exosomes are well established effectors of cell–cell communication. Their role on maturation of embryonic cells located in hippocampus, seat of memory, is unknown. Here we show that ceramide facilitates release of exosomes from HN9.10e cells extending information for cell differentiation to neighboring cells. We found only 38 miRNAs differentially expressed in exosomes derived from ceramide-treated cells in comparison with control cells (including 10 up-regulated and 28 down-regulated). Some overexpressed miRNAs (mmu-let-7f-1-3p, mmu-let-7a-1-3p, mmu-let-7b-3p, mmu-let-7b-5p, mmu-miR-330-3p) regulate genes encoding for protein involved in biological, homeostatic, biosynthetic and small molecule metabolic processes, embryo development and cell differentiation, all phenomena relevant for HN9.10e cell differentiation. Notably, the overexpressed mmu-let-7b-5p miRNA appears to be important for our study based on its ability to regulate thirty-five gene targets involved in many processes including sphingolipid metabolism, sphingolipid-related stimulation of cellular functions and neuronal development. Furthermore, we showed that by incubating embryonic cells with exosomes released under ceramide treatment, some cells acquired an astrocytic phenotype and others a neuronal phenotype. We anticipate our study to be a start point for innovative therapeutic strategies to regulate the release of exosomes useful to stimulate delayed brain development in the newborn and to improve the cognitive decline in neurodegenerative disorders.
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spelling pubmed-103290222023-07-09 Ceramide releases exosomes with a specific miRNA signature for cell differentiation Fiorani, Federico Domenis, Rossana Dalla, Emiliano Cataldi, Samuela Conte, Carmela Mandarano, Martina Sidoni, Angelo Cifù, Adriana Beccari, Tommaso Mirarchi, Alessandra Arcuri, Cataldo Curcio, Francesco Albi, Elisabetta Sci Rep Article Exosomes are well established effectors of cell–cell communication. Their role on maturation of embryonic cells located in hippocampus, seat of memory, is unknown. Here we show that ceramide facilitates release of exosomes from HN9.10e cells extending information for cell differentiation to neighboring cells. We found only 38 miRNAs differentially expressed in exosomes derived from ceramide-treated cells in comparison with control cells (including 10 up-regulated and 28 down-regulated). Some overexpressed miRNAs (mmu-let-7f-1-3p, mmu-let-7a-1-3p, mmu-let-7b-3p, mmu-let-7b-5p, mmu-miR-330-3p) regulate genes encoding for protein involved in biological, homeostatic, biosynthetic and small molecule metabolic processes, embryo development and cell differentiation, all phenomena relevant for HN9.10e cell differentiation. Notably, the overexpressed mmu-let-7b-5p miRNA appears to be important for our study based on its ability to regulate thirty-five gene targets involved in many processes including sphingolipid metabolism, sphingolipid-related stimulation of cellular functions and neuronal development. Furthermore, we showed that by incubating embryonic cells with exosomes released under ceramide treatment, some cells acquired an astrocytic phenotype and others a neuronal phenotype. We anticipate our study to be a start point for innovative therapeutic strategies to regulate the release of exosomes useful to stimulate delayed brain development in the newborn and to improve the cognitive decline in neurodegenerative disorders. Nature Publishing Group UK 2023-07-07 /pmc/articles/PMC10329022/ /pubmed/37419964 http://dx.doi.org/10.1038/s41598-023-38011-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Fiorani, Federico
Domenis, Rossana
Dalla, Emiliano
Cataldi, Samuela
Conte, Carmela
Mandarano, Martina
Sidoni, Angelo
Cifù, Adriana
Beccari, Tommaso
Mirarchi, Alessandra
Arcuri, Cataldo
Curcio, Francesco
Albi, Elisabetta
Ceramide releases exosomes with a specific miRNA signature for cell differentiation
title Ceramide releases exosomes with a specific miRNA signature for cell differentiation
title_full Ceramide releases exosomes with a specific miRNA signature for cell differentiation
title_fullStr Ceramide releases exosomes with a specific miRNA signature for cell differentiation
title_full_unstemmed Ceramide releases exosomes with a specific miRNA signature for cell differentiation
title_short Ceramide releases exosomes with a specific miRNA signature for cell differentiation
title_sort ceramide releases exosomes with a specific mirna signature for cell differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329022/
https://www.ncbi.nlm.nih.gov/pubmed/37419964
http://dx.doi.org/10.1038/s41598-023-38011-1
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