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A single bacterial genus maintains root growth in a complex microbiome
Plants grow within a complex web of species that interact with each other and with the plant(1–10). These interactions are governed by a wide repertoire of chemical signals, and the resulting chemical landscape of the rhizosphere can strongly affect root health and development(7–9,11–18). Here, to u...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329457/ https://www.ncbi.nlm.nih.gov/pubmed/32999461 http://dx.doi.org/10.1038/s41586-020-2778-7 |
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author | Finkel, Omri M. Salas-González, Isai Castrillo, Gabriel Conway, Jonathan M. Law, Theresa F. Teixeira, Paulo José Pereira Lima Wilson, Ellie D. Fitzpatrick, Connor R. Jones, Corbin D. Dangl, Jeffery L. |
author_facet | Finkel, Omri M. Salas-González, Isai Castrillo, Gabriel Conway, Jonathan M. Law, Theresa F. Teixeira, Paulo José Pereira Lima Wilson, Ellie D. Fitzpatrick, Connor R. Jones, Corbin D. Dangl, Jeffery L. |
author_sort | Finkel, Omri M. |
collection | PubMed |
description | Plants grow within a complex web of species that interact with each other and with the plant(1–10). These interactions are governed by a wide repertoire of chemical signals, and the resulting chemical landscape of the rhizosphere can strongly affect root health and development(7–9,11–18). Here, to understand how interactions between microorganisms influence root growth in Arabidopsis, we established a model system for interactions between plants, microorganisms and the environment. We inoculated seedlings with a 185-member bacterial synthetic community, manipulated the abiotic environment and measured bacterial colonization of the plant. This enabled us to classify the synthetic community into four modules of co-occurring strains. We deconstructed the synthetic community on the basis of these modules, and identified interactions between microorganisms that determine root phenotype. These interactions primarily involve a single bacterial genus (Variovorax), which completely reverses the severe inhibition of root growth that is induced by a wide diversity of bacterial strains as well as by the entire 185-member community. We demonstrate that Variovorax manipulates plant hormone levels to balance the effects of our ecologically realistic synthetic root community on root growth. We identify an auxin-degradation operon that is conserved in all available genomes of Variovorax and is necessary and sufficient for the reversion of root growth inhibition. Therefore, metabolic signal interference shapes bacteria–plant communication networks and is essential for maintaining the stereotypic developmental programme of the root. Optimizing the feedbacks that shape chemical interaction networks in the rhizosphere provides a promising ecological strategy for developing more resilient and productive crops. |
format | Online Article Text |
id | pubmed-10329457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-103294572023-07-08 A single bacterial genus maintains root growth in a complex microbiome Finkel, Omri M. Salas-González, Isai Castrillo, Gabriel Conway, Jonathan M. Law, Theresa F. Teixeira, Paulo José Pereira Lima Wilson, Ellie D. Fitzpatrick, Connor R. Jones, Corbin D. Dangl, Jeffery L. Nature Article Plants grow within a complex web of species that interact with each other and with the plant(1–10). These interactions are governed by a wide repertoire of chemical signals, and the resulting chemical landscape of the rhizosphere can strongly affect root health and development(7–9,11–18). Here, to understand how interactions between microorganisms influence root growth in Arabidopsis, we established a model system for interactions between plants, microorganisms and the environment. We inoculated seedlings with a 185-member bacterial synthetic community, manipulated the abiotic environment and measured bacterial colonization of the plant. This enabled us to classify the synthetic community into four modules of co-occurring strains. We deconstructed the synthetic community on the basis of these modules, and identified interactions between microorganisms that determine root phenotype. These interactions primarily involve a single bacterial genus (Variovorax), which completely reverses the severe inhibition of root growth that is induced by a wide diversity of bacterial strains as well as by the entire 185-member community. We demonstrate that Variovorax manipulates plant hormone levels to balance the effects of our ecologically realistic synthetic root community on root growth. We identify an auxin-degradation operon that is conserved in all available genomes of Variovorax and is necessary and sufficient for the reversion of root growth inhibition. Therefore, metabolic signal interference shapes bacteria–plant communication networks and is essential for maintaining the stereotypic developmental programme of the root. Optimizing the feedbacks that shape chemical interaction networks in the rhizosphere provides a promising ecological strategy for developing more resilient and productive crops. 2020-11 2020-09-30 /pmc/articles/PMC10329457/ /pubmed/32999461 http://dx.doi.org/10.1038/s41586-020-2778-7 Text en Reprints and permissions information is available at http://www.nature.com/reprints. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Finkel, Omri M. Salas-González, Isai Castrillo, Gabriel Conway, Jonathan M. Law, Theresa F. Teixeira, Paulo José Pereira Lima Wilson, Ellie D. Fitzpatrick, Connor R. Jones, Corbin D. Dangl, Jeffery L. A single bacterial genus maintains root growth in a complex microbiome |
title | A single bacterial genus maintains root growth in a complex microbiome |
title_full | A single bacterial genus maintains root growth in a complex microbiome |
title_fullStr | A single bacterial genus maintains root growth in a complex microbiome |
title_full_unstemmed | A single bacterial genus maintains root growth in a complex microbiome |
title_short | A single bacterial genus maintains root growth in a complex microbiome |
title_sort | single bacterial genus maintains root growth in a complex microbiome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329457/ https://www.ncbi.nlm.nih.gov/pubmed/32999461 http://dx.doi.org/10.1038/s41586-020-2778-7 |
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