Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance

The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS)...

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Autores principales: Muñoz-Gallego, Irene, Meléndez Carmona, María Ángeles, Martín Higuera, Carmen, Viedma, Esther, Delgado, Rafael, Folgueira, María Dolores
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329708/
https://www.ncbi.nlm.nih.gov/pubmed/37422474
http://dx.doi.org/10.1038/s41598-023-37866-8
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author Muñoz-Gallego, Irene
Meléndez Carmona, María Ángeles
Martín Higuera, Carmen
Viedma, Esther
Delgado, Rafael
Folgueira, María Dolores
author_facet Muñoz-Gallego, Irene
Meléndez Carmona, María Ángeles
Martín Higuera, Carmen
Viedma, Esther
Delgado, Rafael
Folgueira, María Dolores
author_sort Muñoz-Gallego, Irene
collection PubMed
description The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.
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spelling pubmed-103297082023-07-10 Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance Muñoz-Gallego, Irene Meléndez Carmona, María Ángeles Martín Higuera, Carmen Viedma, Esther Delgado, Rafael Folgueira, María Dolores Sci Rep Article The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants. Nature Publishing Group UK 2023-07-08 /pmc/articles/PMC10329708/ /pubmed/37422474 http://dx.doi.org/10.1038/s41598-023-37866-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Muñoz-Gallego, Irene
Meléndez Carmona, María Ángeles
Martín Higuera, Carmen
Viedma, Esther
Delgado, Rafael
Folgueira, María Dolores
Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
title Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
title_full Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
title_fullStr Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
title_full_unstemmed Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
title_short Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
title_sort rapid screening of sars-cov-2 variants, a key tool for pandemic surveillance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329708/
https://www.ncbi.nlm.nih.gov/pubmed/37422474
http://dx.doi.org/10.1038/s41598-023-37866-8
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