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Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark

Ulmus macrocarpa Hance bark (UmHb) has been used as a traditional herbal medicine in East Asia for bone concern diseases for a long time. To find a suitable solvent, we, in this study, compared the efficacy of UmHb water extract and ethanol extract which can inhibit osteoclast differentiation. Compa...

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Autores principales: Jeong, Chanhyeok, Cho, Yeon-Jin, Lee, Yongjin, Wang, Weihong, Park, Kyu-Hyung, Roh, Eun, Lee, Chang Hyung, Son, Young-Jin, Park, Jung Han Yoon, Kang, Heonjoong, Lee, Ki Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330169/
https://www.ncbi.nlm.nih.gov/pubmed/37423923
http://dx.doi.org/10.1038/s41598-023-38208-4
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author Jeong, Chanhyeok
Cho, Yeon-Jin
Lee, Yongjin
Wang, Weihong
Park, Kyu-Hyung
Roh, Eun
Lee, Chang Hyung
Son, Young-Jin
Park, Jung Han Yoon
Kang, Heonjoong
Lee, Ki Won
author_facet Jeong, Chanhyeok
Cho, Yeon-Jin
Lee, Yongjin
Wang, Weihong
Park, Kyu-Hyung
Roh, Eun
Lee, Chang Hyung
Son, Young-Jin
Park, Jung Han Yoon
Kang, Heonjoong
Lee, Ki Won
author_sort Jeong, Chanhyeok
collection PubMed
description Ulmus macrocarpa Hance bark (UmHb) has been used as a traditional herbal medicine in East Asia for bone concern diseases for a long time. To find a suitable solvent, we, in this study, compared the efficacy of UmHb water extract and ethanol extract which can inhibit osteoclast differentiation. Compared with two ethanol extracts (70% and 100% respectively), hydrothermal extracts of UmHb more effectively inhibited receptor activators of nuclear factor κB ligand-induced osteoclast differentiation in murine bone marrow-derived macrophages. We identified for the first time that (2R,3R)-epicatechin-7-O-β-D-apiofuranoside (E7A) is a specific active compound in UmHb hydrothermal extracts through using LC/MS, HPLC, and NMR techniques. In addition, we confirmed through TRAP assay, pit assay, and PCR assay that E7A is a key compound in inhibiting osteoclast differentiation. The optimized condition to obtain E7A-rich UmHb extract was 100 mL/g, 90 °C, pH 5, and 97 min. At this condition, the content of E7A was 26.05 ± 0.96 mg/g extract. Based on TRAP assay, pit assay, PCR, and western blot, the optimized extract of E7A-rich UmHb demonstrated a greater inhibition of osteoclast differentiation compared to unoptimized. These results suggest that E7A would be a good candidate for the prevention and treatment of osteoporosis-related diseases.
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spelling pubmed-103301692023-07-11 Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark Jeong, Chanhyeok Cho, Yeon-Jin Lee, Yongjin Wang, Weihong Park, Kyu-Hyung Roh, Eun Lee, Chang Hyung Son, Young-Jin Park, Jung Han Yoon Kang, Heonjoong Lee, Ki Won Sci Rep Article Ulmus macrocarpa Hance bark (UmHb) has been used as a traditional herbal medicine in East Asia for bone concern diseases for a long time. To find a suitable solvent, we, in this study, compared the efficacy of UmHb water extract and ethanol extract which can inhibit osteoclast differentiation. Compared with two ethanol extracts (70% and 100% respectively), hydrothermal extracts of UmHb more effectively inhibited receptor activators of nuclear factor κB ligand-induced osteoclast differentiation in murine bone marrow-derived macrophages. We identified for the first time that (2R,3R)-epicatechin-7-O-β-D-apiofuranoside (E7A) is a specific active compound in UmHb hydrothermal extracts through using LC/MS, HPLC, and NMR techniques. In addition, we confirmed through TRAP assay, pit assay, and PCR assay that E7A is a key compound in inhibiting osteoclast differentiation. The optimized condition to obtain E7A-rich UmHb extract was 100 mL/g, 90 °C, pH 5, and 97 min. At this condition, the content of E7A was 26.05 ± 0.96 mg/g extract. Based on TRAP assay, pit assay, PCR, and western blot, the optimized extract of E7A-rich UmHb demonstrated a greater inhibition of osteoclast differentiation compared to unoptimized. These results suggest that E7A would be a good candidate for the prevention and treatment of osteoporosis-related diseases. Nature Publishing Group UK 2023-07-09 /pmc/articles/PMC10330169/ /pubmed/37423923 http://dx.doi.org/10.1038/s41598-023-38208-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jeong, Chanhyeok
Cho, Yeon-Jin
Lee, Yongjin
Wang, Weihong
Park, Kyu-Hyung
Roh, Eun
Lee, Chang Hyung
Son, Young-Jin
Park, Jung Han Yoon
Kang, Heonjoong
Lee, Ki Won
Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark
title Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark
title_full Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark
title_fullStr Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark
title_full_unstemmed Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark
title_short Discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-O-β-D-apiofuranoside from Ulmus macrocarpa Hance bark
title_sort discovery and optimized extraction of the anti-osteoclastic agent epicatechin-7-o-β-d-apiofuranoside from ulmus macrocarpa hance bark
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330169/
https://www.ncbi.nlm.nih.gov/pubmed/37423923
http://dx.doi.org/10.1038/s41598-023-38208-4
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