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Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy

Astrocytes are highly heterogeneous and involved in different aspects of fundamental functions in the central nervous system (CNS). However, whether and how this heterogeneous population of cells reacts to the pathophysiological challenge is not well understood. To investigate the response status of...

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Autores principales: Li, Jie, Wang, Pengjun, Wang, Lu-Yang, Wu, Yaqin, Wang, Jiping, Yu, Dongzhen, Chen, Zhengnong, Shi, Haibo, Yin, Shankai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330711/
https://www.ncbi.nlm.nih.gov/pubmed/37434761
http://dx.doi.org/10.3389/fnins.2023.1146147
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author Li, Jie
Wang, Pengjun
Wang, Lu-Yang
Wu, Yaqin
Wang, Jiping
Yu, Dongzhen
Chen, Zhengnong
Shi, Haibo
Yin, Shankai
author_facet Li, Jie
Wang, Pengjun
Wang, Lu-Yang
Wu, Yaqin
Wang, Jiping
Yu, Dongzhen
Chen, Zhengnong
Shi, Haibo
Yin, Shankai
author_sort Li, Jie
collection PubMed
description Astrocytes are highly heterogeneous and involved in different aspects of fundamental functions in the central nervous system (CNS). However, whether and how this heterogeneous population of cells reacts to the pathophysiological challenge is not well understood. To investigate the response status of astrocytes in the medial vestibular nucleus (MVN) after vestibular loss, we examined the subtypes of astrocytes in MVN using single-cell sequencing technology in a unilateral labyrinthectomy mouse model. We discovered four subtypes of astrocytes in the MVN with each displaying unique gene expression profiles. After unilateral labyrinthectomy, the proportion of the astrocytic subtypes and their transcriptional features on the ipsilateral side of the MVN differ significantly from those on the contralateral side. With new markers to detect and classify the subtypes of astrocytes in the MVN, our findings implicate potential roles of the adaptive changes of astrocyte subtypes in the early vestibular compensation following peripheral vestibular damage to reverse behavioral deficits.
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spelling pubmed-103307112023-07-11 Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy Li, Jie Wang, Pengjun Wang, Lu-Yang Wu, Yaqin Wang, Jiping Yu, Dongzhen Chen, Zhengnong Shi, Haibo Yin, Shankai Front Neurosci Neuroscience Astrocytes are highly heterogeneous and involved in different aspects of fundamental functions in the central nervous system (CNS). However, whether and how this heterogeneous population of cells reacts to the pathophysiological challenge is not well understood. To investigate the response status of astrocytes in the medial vestibular nucleus (MVN) after vestibular loss, we examined the subtypes of astrocytes in MVN using single-cell sequencing technology in a unilateral labyrinthectomy mouse model. We discovered four subtypes of astrocytes in the MVN with each displaying unique gene expression profiles. After unilateral labyrinthectomy, the proportion of the astrocytic subtypes and their transcriptional features on the ipsilateral side of the MVN differ significantly from those on the contralateral side. With new markers to detect and classify the subtypes of astrocytes in the MVN, our findings implicate potential roles of the adaptive changes of astrocyte subtypes in the early vestibular compensation following peripheral vestibular damage to reverse behavioral deficits. Frontiers Media S.A. 2023-06-26 /pmc/articles/PMC10330711/ /pubmed/37434761 http://dx.doi.org/10.3389/fnins.2023.1146147 Text en Copyright © 2023 Li, Wang, Wang, Wu, Wang, Yu, Chen, Shi and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Li, Jie
Wang, Pengjun
Wang, Lu-Yang
Wu, Yaqin
Wang, Jiping
Yu, Dongzhen
Chen, Zhengnong
Shi, Haibo
Yin, Shankai
Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
title Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
title_full Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
title_fullStr Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
title_full_unstemmed Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
title_short Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
title_sort redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330711/
https://www.ncbi.nlm.nih.gov/pubmed/37434761
http://dx.doi.org/10.3389/fnins.2023.1146147
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