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RNA extraction protocol from low-biomass bacterial Nitrosomonas europaea and Nitrobacter winogradskyi cultures for whole transcriptome studies
RNA-sequencing for whole transcriptome analysis requires high-quality RNA in adequate amounts, which can be difficult to generate with low-biomass-producing bacteria where sample volume is limited. We present an RNA extraction protocol for low-biomass-producing autotrophic bacteria Nitrosomonas euro...
Autores principales: | Verbeelen, Tom, Van Houdt, Rob, Leys, Natalie, Ganigué, Ramon, Mastroleo, Felice |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331297/ https://www.ncbi.nlm.nih.gov/pubmed/37347668 http://dx.doi.org/10.1016/j.xpro.2023.102358 |
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