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IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils
Interleukin (IL)-4 and IL-13 are related cytokines with well-known specific roles in type 2 immune response. However, their effects on neutrophils are not completely understood. For this, we studied human primary neutrophil responses to IL-4 and IL-13. Neutrophils are dose-dependently responsive to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331420/ https://www.ncbi.nlm.nih.gov/pubmed/36809715 http://dx.doi.org/10.1016/j.cyto.2023.156159 |
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author | Kummola, Laura Salomaa, Tanja Ortutay, Zsuzsanna Savan, Ram Young, Howard A. Junttila, Ilkka S. |
author_facet | Kummola, Laura Salomaa, Tanja Ortutay, Zsuzsanna Savan, Ram Young, Howard A. Junttila, Ilkka S. |
author_sort | Kummola, Laura |
collection | PubMed |
description | Interleukin (IL)-4 and IL-13 are related cytokines with well-known specific roles in type 2 immune response. However, their effects on neutrophils are not completely understood. For this, we studied human primary neutrophil responses to IL-4 and IL-13. Neutrophils are dose-dependently responsive to both IL-4 and IL-13 as indicated by signal transducer and activator of transcription 6 (STAT6) phosphorylation upon stimulation, with IL-4 being more potent inducer of STAT6. IL-4-, IL-13- and Interferon (IFN)-γ-stimulated gene expression in highly purified human neutrophils induced both overlapping and unique gene expression in highly purified human neutrophils. IL-4 and IL-13 specifically regulate several immune-related genes, including IL-10, tumor necrosis factor (TNF) and leukemia inhibitory factor (LIF), while type1 immune response-related IFN-γ induced gene expression related for example, to intracellular infections. In analysis of neutrophil metabolic responses, oxygen independent glycolysis was specifically regulated by IL-4, but not by IL-13 or IFN-γ, suggesting specific role for type I IL-4 receptor in this process. Our results provide a comprehensive analysis of IL-4, IL-13 and IFN-γ -induced gene expression in neutrophils while also addressing cytokine-mediated metabolic changes in neutrophils. |
format | Online Article Text |
id | pubmed-10331420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-103314202023-07-10 IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils Kummola, Laura Salomaa, Tanja Ortutay, Zsuzsanna Savan, Ram Young, Howard A. Junttila, Ilkka S. Cytokine Article Interleukin (IL)-4 and IL-13 are related cytokines with well-known specific roles in type 2 immune response. However, their effects on neutrophils are not completely understood. For this, we studied human primary neutrophil responses to IL-4 and IL-13. Neutrophils are dose-dependently responsive to both IL-4 and IL-13 as indicated by signal transducer and activator of transcription 6 (STAT6) phosphorylation upon stimulation, with IL-4 being more potent inducer of STAT6. IL-4-, IL-13- and Interferon (IFN)-γ-stimulated gene expression in highly purified human neutrophils induced both overlapping and unique gene expression in highly purified human neutrophils. IL-4 and IL-13 specifically regulate several immune-related genes, including IL-10, tumor necrosis factor (TNF) and leukemia inhibitory factor (LIF), while type1 immune response-related IFN-γ induced gene expression related for example, to intracellular infections. In analysis of neutrophil metabolic responses, oxygen independent glycolysis was specifically regulated by IL-4, but not by IL-13 or IFN-γ, suggesting specific role for type I IL-4 receptor in this process. Our results provide a comprehensive analysis of IL-4, IL-13 and IFN-γ -induced gene expression in neutrophils while also addressing cytokine-mediated metabolic changes in neutrophils. 2023-04 2023-02-19 /pmc/articles/PMC10331420/ /pubmed/36809715 http://dx.doi.org/10.1016/j.cyto.2023.156159 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Kummola, Laura Salomaa, Tanja Ortutay, Zsuzsanna Savan, Ram Young, Howard A. Junttila, Ilkka S. IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils |
title | IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils |
title_full | IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils |
title_fullStr | IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils |
title_full_unstemmed | IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils |
title_short | IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils |
title_sort | il-4, il-13 and ifn-γ -induced genes in highly purified human neutrophils |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331420/ https://www.ncbi.nlm.nih.gov/pubmed/36809715 http://dx.doi.org/10.1016/j.cyto.2023.156159 |
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