Cargando…

Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus

Novel phenotypes can come about through a variety of mechanisms including standing genetic variation from a founding population. Cave animals are an excellent system in which to study the evolution of novel phenotypes such as loss of pigmentation and eyes. Asellus aquaticus is a freshwater isopod cr...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodas, Lizet R., Sarbu, Serban M., Bancila, Raluca, Price, Devon, Fišer, Žiga, Protas, Meredith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331845/
https://www.ncbi.nlm.nih.gov/pubmed/36755467
http://dx.doi.org/10.1111/ede.12428
_version_ 1785070329956139008
author Rodas, Lizet R.
Sarbu, Serban M.
Bancila, Raluca
Price, Devon
Fišer, Žiga
Protas, Meredith
author_facet Rodas, Lizet R.
Sarbu, Serban M.
Bancila, Raluca
Price, Devon
Fišer, Žiga
Protas, Meredith
author_sort Rodas, Lizet R.
collection PubMed
description Novel phenotypes can come about through a variety of mechanisms including standing genetic variation from a founding population. Cave animals are an excellent system in which to study the evolution of novel phenotypes such as loss of pigmentation and eyes. Asellus aquaticus is a freshwater isopod crustacean found in Europe and has both a surface and a cave ecomorph which vary in multiple phenotypic traits. An orange eye phenotype was previously revealed by F(2) crosses and backcrosses to the cave parent within two examined Slovenian cave populations. Complete loss of pigmentation, both in eye and body, is epistatic to the orange eye phenotype and therefore the orange eye phenotype is hidden within the cave populations. Our goal was to investigate the origin of the orange eye alleles within the Slovenian cave populations by examining A. aquaticus individuals from Slovenian and Romanian surface populations and Asellus aquaticus infernus individuals from a Romanian cave population. We found orange eye individuals present in lab raised surface populations of A. aquaticus from both Slovenia and Romania. Using a mapping approach with crosses between individuals of two surface populations, we found that the region known to be responsible for the orange eye phenotype within the two previously examined Slovenian cave populations was also responsible within both the Slovenian and the Romanian surface populations. Complementation crosses between orange eye Slovenian and orange eye Romanian surface individuals suggest that the same gene is responsible for the orange eye phenotype in both surface populations. Additionally, we observed a low frequency phenotype of eye loss in crosses generated between the two surface populations and also in the Romanian surface population. Finally, in a cave population from Romania, A. aquaticus infernus, we found that the same region is also responsible for the orange eye phenotype as the Slovenian cave populations and the Slovenian and Romanian surface populations. Therefore, we present evidence that variation present in the cave populations could originate from standing variation present in the surface populations and/or transgressive hybridization of different surface phylogenetic lineages rather than de novo mutations.
format Online
Article
Text
id pubmed-10331845
institution National Center for Biotechnology Information
language English
publishDate 2023
record_format MEDLINE/PubMed
spelling pubmed-103318452023-07-10 Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus Rodas, Lizet R. Sarbu, Serban M. Bancila, Raluca Price, Devon Fišer, Žiga Protas, Meredith Evol Dev Article Novel phenotypes can come about through a variety of mechanisms including standing genetic variation from a founding population. Cave animals are an excellent system in which to study the evolution of novel phenotypes such as loss of pigmentation and eyes. Asellus aquaticus is a freshwater isopod crustacean found in Europe and has both a surface and a cave ecomorph which vary in multiple phenotypic traits. An orange eye phenotype was previously revealed by F(2) crosses and backcrosses to the cave parent within two examined Slovenian cave populations. Complete loss of pigmentation, both in eye and body, is epistatic to the orange eye phenotype and therefore the orange eye phenotype is hidden within the cave populations. Our goal was to investigate the origin of the orange eye alleles within the Slovenian cave populations by examining A. aquaticus individuals from Slovenian and Romanian surface populations and Asellus aquaticus infernus individuals from a Romanian cave population. We found orange eye individuals present in lab raised surface populations of A. aquaticus from both Slovenia and Romania. Using a mapping approach with crosses between individuals of two surface populations, we found that the region known to be responsible for the orange eye phenotype within the two previously examined Slovenian cave populations was also responsible within both the Slovenian and the Romanian surface populations. Complementation crosses between orange eye Slovenian and orange eye Romanian surface individuals suggest that the same gene is responsible for the orange eye phenotype in both surface populations. Additionally, we observed a low frequency phenotype of eye loss in crosses generated between the two surface populations and also in the Romanian surface population. Finally, in a cave population from Romania, A. aquaticus infernus, we found that the same region is also responsible for the orange eye phenotype as the Slovenian cave populations and the Slovenian and Romanian surface populations. Therefore, we present evidence that variation present in the cave populations could originate from standing variation present in the surface populations and/or transgressive hybridization of different surface phylogenetic lineages rather than de novo mutations. 2023-03 2023-02-08 /pmc/articles/PMC10331845/ /pubmed/36755467 http://dx.doi.org/10.1111/ede.12428 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Article
Rodas, Lizet R.
Sarbu, Serban M.
Bancila, Raluca
Price, Devon
Fišer, Žiga
Protas, Meredith
Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus
title Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus
title_full Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus
title_fullStr Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus
title_full_unstemmed Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus
title_short Standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, Asellus aquaticus
title_sort standing genetic variation as a potential mechanism of novel cave phenotype evolution in the freshwater isopod, asellus aquaticus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331845/
https://www.ncbi.nlm.nih.gov/pubmed/36755467
http://dx.doi.org/10.1111/ede.12428
work_keys_str_mv AT rodaslizetr standinggeneticvariationasapotentialmechanismofnovelcavephenotypeevolutioninthefreshwaterisopodasellusaquaticus
AT sarbuserbanm standinggeneticvariationasapotentialmechanismofnovelcavephenotypeevolutioninthefreshwaterisopodasellusaquaticus
AT bancilaraluca standinggeneticvariationasapotentialmechanismofnovelcavephenotypeevolutioninthefreshwaterisopodasellusaquaticus
AT pricedevon standinggeneticvariationasapotentialmechanismofnovelcavephenotypeevolutioninthefreshwaterisopodasellusaquaticus
AT fiserziga standinggeneticvariationasapotentialmechanismofnovelcavephenotypeevolutioninthefreshwaterisopodasellusaquaticus
AT protasmeredith standinggeneticvariationasapotentialmechanismofnovelcavephenotypeevolutioninthefreshwaterisopodasellusaquaticus