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Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance

Species belonging to the Vernonia (Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemi...

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Autores principales: Cong, Pham Van, Anh, Hoang Le Tuan, Vinh, Le Ba, Han, Yoo Kyong, Trung, Nguyen Quang, Minh, Bui Quang, Duc, Ngo Viet, Ngoc, Tran Minh, Hien, Nguyen Thi Thu, Manh, Hoang Duc, Lien, Le Thi, Lee, Ki Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331943/
https://www.ncbi.nlm.nih.gov/pubmed/36908274
http://dx.doi.org/10.4014/jmb.2212.12040
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author Cong, Pham Van
Anh, Hoang Le Tuan
Vinh, Le Ba
Han, Yoo Kyong
Trung, Nguyen Quang
Minh, Bui Quang
Duc, Ngo Viet
Ngoc, Tran Minh
Hien, Nguyen Thi Thu
Manh, Hoang Duc
Lien, Le Thi
Lee, Ki Yong
author_facet Cong, Pham Van
Anh, Hoang Le Tuan
Vinh, Le Ba
Han, Yoo Kyong
Trung, Nguyen Quang
Minh, Bui Quang
Duc, Ngo Viet
Ngoc, Tran Minh
Hien, Nguyen Thi Thu
Manh, Hoang Duc
Lien, Le Thi
Lee, Ki Yong
author_sort Cong, Pham Van
collection PubMed
description Species belonging to the Vernonia (Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemical components. A phytochemical investigation resulted in the separation of three new stigmastane-type steroidal saponins (1 – 3), designated as vernogratiosides A–C, from whole plants of V. gratiosa. Their structures were elucidated based on infrared spectroscopy (IR), one-dimensional (1D) and two-dimensional nuclear magnetic resonance (2D NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and electronic circular dichroism analyses (ECD), as well as chemical reactivity. Molecular docking analysis of representative saponins with α-glucosidase inhibitory activity was performed. Additionally, the intended substances were tested for their ability to inhibit α-glucosidase activity in a laboratory setting. The results suggested that stigmastane-type steroidal saponins from V. gratiosa are promising candidate antidiabetic agents.
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spelling pubmed-103319432023-07-11 Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance Cong, Pham Van Anh, Hoang Le Tuan Vinh, Le Ba Han, Yoo Kyong Trung, Nguyen Quang Minh, Bui Quang Duc, Ngo Viet Ngoc, Tran Minh Hien, Nguyen Thi Thu Manh, Hoang Duc Lien, Le Thi Lee, Ki Yong J Microbiol Biotechnol Research article Species belonging to the Vernonia (Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemical components. A phytochemical investigation resulted in the separation of three new stigmastane-type steroidal saponins (1 – 3), designated as vernogratiosides A–C, from whole plants of V. gratiosa. Their structures were elucidated based on infrared spectroscopy (IR), one-dimensional (1D) and two-dimensional nuclear magnetic resonance (2D NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and electronic circular dichroism analyses (ECD), as well as chemical reactivity. Molecular docking analysis of representative saponins with α-glucosidase inhibitory activity was performed. Additionally, the intended substances were tested for their ability to inhibit α-glucosidase activity in a laboratory setting. The results suggested that stigmastane-type steroidal saponins from V. gratiosa are promising candidate antidiabetic agents. The Korean Society for Microbiology and Biotechnology 2023-06-28 2023-03-06 /pmc/articles/PMC10331943/ /pubmed/36908274 http://dx.doi.org/10.4014/jmb.2212.12040 Text en Copyright © 2023 by the authors. Licensee KMB https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
spellingShingle Research article
Cong, Pham Van
Anh, Hoang Le Tuan
Vinh, Le Ba
Han, Yoo Kyong
Trung, Nguyen Quang
Minh, Bui Quang
Duc, Ngo Viet
Ngoc, Tran Minh
Hien, Nguyen Thi Thu
Manh, Hoang Duc
Lien, Le Thi
Lee, Ki Yong
Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
title Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
title_full Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
title_fullStr Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
title_full_unstemmed Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
title_short Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
title_sort alpha-glucosidase inhibitory activity of saponins isolated from vernonia gratiosa hance
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10331943/
https://www.ncbi.nlm.nih.gov/pubmed/36908274
http://dx.doi.org/10.4014/jmb.2212.12040
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