METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity

The epigenetic modification of the N6-methyladenosine (m(6)A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m(6)A modifications are increased in PK-15 cel...

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Autores principales: Li, Chen, Wu, Xiaoyan, Yang, Ying, Shi, Jianli, Wang, Shuo, Li, Jiaxin, Tian, Yao, Liu, Chang, Han, Hong, Xu, Shaojian, Han, Xianjie, Ma, Yingru, Zheng, Limei, Li, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
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Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10332184/
https://www.ncbi.nlm.nih.gov/pubmed/37418592
http://dx.doi.org/10.1080/21505594.2023.2232910
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author Li, Chen
Wu, Xiaoyan
Yang, Ying
Shi, Jianli
Wang, Shuo
Li, Jiaxin
Tian, Yao
Liu, Chang
Han, Hong
Xu, Shaojian
Han, Xianjie
Ma, Yingru
Zheng, Limei
Li, Jun
author_facet Li, Chen
Wu, Xiaoyan
Yang, Ying
Shi, Jianli
Wang, Shuo
Li, Jiaxin
Tian, Yao
Liu, Chang
Han, Hong
Xu, Shaojian
Han, Xianjie
Ma, Yingru
Zheng, Limei
Li, Jun
author_sort Li, Chen
collection PubMed
description The epigenetic modification of the N6-methyladenosine (m(6)A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m(6)A modifications are increased in PK-15 cells after PCV2 infection. In particular, PCV2 infection could increase the expression of methyltransferase METTL14 and demethylase FTO. Moreover, interfering with METTL14 accumulation reduced the m(6)A methylation level and virus reproduction, whereas depleting the FTO demethylase enhanced the m(6)A methylation level and stimulated virus reproduction. Besides, we showed that METTL14 and FTO regulate PCV2 replication by affecting the process of miRNA maturity, especially the miRNA-30a-5p. Taken together, our results demonstrated that the m(6)A modification positively affects PCV2 replication and the role of m(6)A modification in the replication mechanism of the PCV2 virus provides a new idea for the prevention and control of the PCV2.
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spelling pubmed-103321842023-07-11 METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity Li, Chen Wu, Xiaoyan Yang, Ying Shi, Jianli Wang, Shuo Li, Jiaxin Tian, Yao Liu, Chang Han, Hong Xu, Shaojian Han, Xianjie Ma, Yingru Zheng, Limei Li, Jun Virulence Research Paper The epigenetic modification of the N6-methyladenosine (m(6)A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m(6)A modifications are increased in PK-15 cells after PCV2 infection. In particular, PCV2 infection could increase the expression of methyltransferase METTL14 and demethylase FTO. Moreover, interfering with METTL14 accumulation reduced the m(6)A methylation level and virus reproduction, whereas depleting the FTO demethylase enhanced the m(6)A methylation level and stimulated virus reproduction. Besides, we showed that METTL14 and FTO regulate PCV2 replication by affecting the process of miRNA maturity, especially the miRNA-30a-5p. Taken together, our results demonstrated that the m(6)A modification positively affects PCV2 replication and the role of m(6)A modification in the replication mechanism of the PCV2 virus provides a new idea for the prevention and control of the PCV2. Taylor & Francis 2023-07-07 /pmc/articles/PMC10332184/ /pubmed/37418592 http://dx.doi.org/10.1080/21505594.2023.2232910 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Paper
Li, Chen
Wu, Xiaoyan
Yang, Ying
Shi, Jianli
Wang, Shuo
Li, Jiaxin
Tian, Yao
Liu, Chang
Han, Hong
Xu, Shaojian
Han, Xianjie
Ma, Yingru
Zheng, Limei
Li, Jun
METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
title METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
title_full METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
title_fullStr METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
title_full_unstemmed METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
title_short METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
title_sort mettl14 and fto mediated m(6)a modification regulate pcv2 replication by affecting mir-30a-5p maturity
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10332184/
https://www.ncbi.nlm.nih.gov/pubmed/37418592
http://dx.doi.org/10.1080/21505594.2023.2232910
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