Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample

A 38-year-old man was taken to hospital with generalized clonic seizure. Brain magnetic resonance imaging (MRI) showed multiple ring-enhancing lesions centered in the left frontoparietal lobe. A histopathological examination of a brain biopsy sample revealed granulomatous lesions with caseous necros...

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Autores principales: Yamamoto, Morihiro, Manabe, Tatsuo, Yokokawa, Kazuki, Tsuzaka, Kazufumi, Yamada, Minoru, Miyanaga, Rei, Saito, Taro, Yamamoto, Daisuke, Matsumura, Akihiro, Suzuki, Syuuichirou, Hisahara, Shin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Internal Medicine 2022
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10332954/
https://www.ncbi.nlm.nih.gov/pubmed/36328574
http://dx.doi.org/10.2169/internalmedicine.0356-22
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author Yamamoto, Morihiro
Manabe, Tatsuo
Yokokawa, Kazuki
Tsuzaka, Kazufumi
Yamada, Minoru
Miyanaga, Rei
Saito, Taro
Yamamoto, Daisuke
Matsumura, Akihiro
Suzuki, Syuuichirou
Hisahara, Shin
author_facet Yamamoto, Morihiro
Manabe, Tatsuo
Yokokawa, Kazuki
Tsuzaka, Kazufumi
Yamada, Minoru
Miyanaga, Rei
Saito, Taro
Yamamoto, Daisuke
Matsumura, Akihiro
Suzuki, Syuuichirou
Hisahara, Shin
author_sort Yamamoto, Morihiro
collection PubMed
description A 38-year-old man was taken to hospital with generalized clonic seizure. Brain magnetic resonance imaging (MRI) showed multiple ring-enhancing lesions centered in the left frontoparietal lobe. A histopathological examination of a brain biopsy sample revealed granulomatous lesions with caseous necrosis. We extracted DNA from a formalin-fixed paraffin-embedded (FFPE) brain specimen, and nested polymerase chain reaction (PCR) of the DNA sample detected the Mycobacterium tuberculosis-specific insertion sequence IS6110. The lesions worsened after anti-tuberculosis drugs were administered, which we considered to be a paradoxical response and continued treatment. A genetic diagnosis of M. tuberculosis using FFPE specimens is useful for diagnosing tuberculoma.
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spelling pubmed-103329542023-07-12 Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample Yamamoto, Morihiro Manabe, Tatsuo Yokokawa, Kazuki Tsuzaka, Kazufumi Yamada, Minoru Miyanaga, Rei Saito, Taro Yamamoto, Daisuke Matsumura, Akihiro Suzuki, Syuuichirou Hisahara, Shin Intern Med Case Report A 38-year-old man was taken to hospital with generalized clonic seizure. Brain magnetic resonance imaging (MRI) showed multiple ring-enhancing lesions centered in the left frontoparietal lobe. A histopathological examination of a brain biopsy sample revealed granulomatous lesions with caseous necrosis. We extracted DNA from a formalin-fixed paraffin-embedded (FFPE) brain specimen, and nested polymerase chain reaction (PCR) of the DNA sample detected the Mycobacterium tuberculosis-specific insertion sequence IS6110. The lesions worsened after anti-tuberculosis drugs were administered, which we considered to be a paradoxical response and continued treatment. A genetic diagnosis of M. tuberculosis using FFPE specimens is useful for diagnosing tuberculoma. The Japanese Society of Internal Medicine 2022-11-02 2023-06-15 /pmc/articles/PMC10332954/ /pubmed/36328574 http://dx.doi.org/10.2169/internalmedicine.0356-22 Text en Copyright © 2023 by The Japanese Society of Internal Medicine https://creativecommons.org/licenses/by-nc-nd/4.0/The Internal Medicine is an Open Access journal distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Case Report
Yamamoto, Morihiro
Manabe, Tatsuo
Yokokawa, Kazuki
Tsuzaka, Kazufumi
Yamada, Minoru
Miyanaga, Rei
Saito, Taro
Yamamoto, Daisuke
Matsumura, Akihiro
Suzuki, Syuuichirou
Hisahara, Shin
Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample
title Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample
title_full Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample
title_fullStr Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample
title_full_unstemmed Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample
title_short Cerebral Tuberculoma Diagnosed by Nested Polymerase Chain Reaction of a Formalin-fixed Paraffin-embedded Brain Biopsy Sample
title_sort cerebral tuberculoma diagnosed by nested polymerase chain reaction of a formalin-fixed paraffin-embedded brain biopsy sample
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10332954/
https://www.ncbi.nlm.nih.gov/pubmed/36328574
http://dx.doi.org/10.2169/internalmedicine.0356-22
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