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In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study

In this proof‐of‐concept study of twenty participants, we sought to determine if a DACC (Dialkylcarbamoyl chloride)‐coated mesh dressing demonstrates an ability to adhere biofilm when placed on Diabetes Related Foot Ulcers (DRFUs) with chronic infection. The study also sought to determine if removal...

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Autores principales: Malone, Matthew, Radzieta, Michael, Schwarzer, Saskia, Walker, Amy, Bradley, Justin, Jensen, Slade O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333049/
https://www.ncbi.nlm.nih.gov/pubmed/36567138
http://dx.doi.org/10.1111/iwj.14054
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author Malone, Matthew
Radzieta, Michael
Schwarzer, Saskia
Walker, Amy
Bradley, Justin
Jensen, Slade O.
author_facet Malone, Matthew
Radzieta, Michael
Schwarzer, Saskia
Walker, Amy
Bradley, Justin
Jensen, Slade O.
author_sort Malone, Matthew
collection PubMed
description In this proof‐of‐concept study of twenty participants, we sought to determine if a DACC (Dialkylcarbamoyl chloride)‐coated mesh dressing demonstrates an ability to adhere biofilm when placed on Diabetes Related Foot Ulcers (DRFUs) with chronic infection. The study also sought to determine if removal of the DACC‐coated mesh dressings contributes to reducing the total number of bacteria in DRFUs, by exploring the total microbial loads, microbial community composition, and diversity. Standard of care was provided in addition to the application of DACC or DACC hydrogel every three days for a total of two weeks. Wound swabs, tissue curettage, and soiled dressings were collected pre and post‐treatment. Tissue specimens obtained pre‐treatment were analysed with scanning electron microscopy (SEM) and peptide nucleic acid fluorescent in situ hybridisation (PNA‐FISH) with confocal laser scanning microscopy and confirmed the presence of biofilm in all DRFUs. SEM confirmed the presence of biofilms readily adhered to soiled DACC‐coated mesh dressings pre‐ and post‐treatment in all participants. Real‐time quantitative polymerase chain reaction (qPCR) demonstrated the mean total microbial load of DRFUs in 20 participants did not change after two weeks of therapy (pre‐treatment = 4.31 Log10 16 S copies (±0.8) versus end of treatment = 4.32 Log10 16 S copies (±0.9), P = .96, 95% CI −0.56 to 0.5). 16 S sequencing has shown the microbial composition of DACC dressings and wound swabs pre‐ and post‐treatment remained similar (DACC; R = −.047, P = .98, Swab; R = −.04, P = .86), indicating the microbial communities originate from the ulcer. Biofilms adhere to DACC‐coated mesh dressings; however, this may not reduce the total microbial load present within DRFU tissue. Wound dressings for use in hard‐to‐heal wounds should be used as an adjunct to a good standard of care which includes debridement and wound bed preparation.
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spelling pubmed-103330492023-07-12 In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study Malone, Matthew Radzieta, Michael Schwarzer, Saskia Walker, Amy Bradley, Justin Jensen, Slade O. Int Wound J Original Articles In this proof‐of‐concept study of twenty participants, we sought to determine if a DACC (Dialkylcarbamoyl chloride)‐coated mesh dressing demonstrates an ability to adhere biofilm when placed on Diabetes Related Foot Ulcers (DRFUs) with chronic infection. The study also sought to determine if removal of the DACC‐coated mesh dressings contributes to reducing the total number of bacteria in DRFUs, by exploring the total microbial loads, microbial community composition, and diversity. Standard of care was provided in addition to the application of DACC or DACC hydrogel every three days for a total of two weeks. Wound swabs, tissue curettage, and soiled dressings were collected pre and post‐treatment. Tissue specimens obtained pre‐treatment were analysed with scanning electron microscopy (SEM) and peptide nucleic acid fluorescent in situ hybridisation (PNA‐FISH) with confocal laser scanning microscopy and confirmed the presence of biofilm in all DRFUs. SEM confirmed the presence of biofilms readily adhered to soiled DACC‐coated mesh dressings pre‐ and post‐treatment in all participants. Real‐time quantitative polymerase chain reaction (qPCR) demonstrated the mean total microbial load of DRFUs in 20 participants did not change after two weeks of therapy (pre‐treatment = 4.31 Log10 16 S copies (±0.8) versus end of treatment = 4.32 Log10 16 S copies (±0.9), P = .96, 95% CI −0.56 to 0.5). 16 S sequencing has shown the microbial composition of DACC dressings and wound swabs pre‐ and post‐treatment remained similar (DACC; R = −.047, P = .98, Swab; R = −.04, P = .86), indicating the microbial communities originate from the ulcer. Biofilms adhere to DACC‐coated mesh dressings; however, this may not reduce the total microbial load present within DRFU tissue. Wound dressings for use in hard‐to‐heal wounds should be used as an adjunct to a good standard of care which includes debridement and wound bed preparation. Blackwell Publishing Ltd 2022-12-25 /pmc/articles/PMC10333049/ /pubmed/36567138 http://dx.doi.org/10.1111/iwj.14054 Text en © 2022 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Malone, Matthew
Radzieta, Michael
Schwarzer, Saskia
Walker, Amy
Bradley, Justin
Jensen, Slade O.
In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study
title In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study
title_full In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study
title_fullStr In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study
title_full_unstemmed In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study
title_short In vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: A proof of concept study
title_sort in vivo observations of biofilm adhering to a dialkylcarbamoyl chloride‐coated mesh dressing when applied to diabetes‐related foot ulcers: a proof of concept study
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333049/
https://www.ncbi.nlm.nih.gov/pubmed/36567138
http://dx.doi.org/10.1111/iwj.14054
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