The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments

Gene editing is a promising alternative to traditional breeding for the generation of new mushroom strains. However, the current approach frequently uses Cas9-plasmid DNA to facilitate mushroom gene editing, which can leave residual foreign DNA in the chromosomal DNA raising concerns regarding genet...

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Detalles Bibliográficos
Autores principales: Eom, Hyerang, Choi, Yeon-Jae, Nandre, Rutuja, Han, Hui-Gang, Kim, Sinil, Kim, Minseek, Oh, Youn-Lee, Nakazawa, Takehito, Honda, Yoichi, Ro, Hyeon-Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333205/
https://www.ncbi.nlm.nih.gov/pubmed/37429890
http://dx.doi.org/10.1038/s41598-023-38331-2

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