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Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics

Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are important...

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Autores principales: Piri-Gharaghie, Tohid, Ghajari, Ghazal, Tolou-Shikhzadeh-Yazdi, Shakiba, Aghassizadeh-Sherbaf, Mona, Khorsand-Dehkordi, Sahar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333334/
https://www.ncbi.nlm.nih.gov/pubmed/37430109
http://dx.doi.org/10.1038/s41598-023-38374-5
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author Piri-Gharaghie, Tohid
Ghajari, Ghazal
Tolou-Shikhzadeh-Yazdi, Shakiba
Aghassizadeh-Sherbaf, Mona
Khorsand-Dehkordi, Sahar
author_facet Piri-Gharaghie, Tohid
Ghajari, Ghazal
Tolou-Shikhzadeh-Yazdi, Shakiba
Aghassizadeh-Sherbaf, Mona
Khorsand-Dehkordi, Sahar
author_sort Piri-Gharaghie, Tohid
collection PubMed
description Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, we looked at the distribution of the pathogenicity cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antimicrobial resistance. Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 raw poultry specimens. Disk diffusion and Multiplex-PCR were used to investigate antimicrobial resistance and genotyping patterns, separately. H. pylori was found in 20 of 320 (6.25%) raw poultry samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas the fewest was found in Goose and Quebec (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were greatest in H. pylori isolates. The percentage of H. pylori isolates with a MAR value of more than 0.2 was 17/20 (85%). The most prevalent genotypes discovered were VacA s1a (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically discovered genotype patterns were s1am1a (45%), s2m1a (45%), and s2m2 (30%). BabA2, OipA + , and OipA− genotypes were found in 40%, 30%, and 30% of the population. In summary, the poultry flesh was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria implies a serious public health concern about raw poultry eating. In the future, researchers should look into H. pylori's resistance to multiple antibacterial drugs in Iran.
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spelling pubmed-103333342023-07-12 Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics Piri-Gharaghie, Tohid Ghajari, Ghazal Tolou-Shikhzadeh-Yazdi, Shakiba Aghassizadeh-Sherbaf, Mona Khorsand-Dehkordi, Sahar Sci Rep Article Even though Helicobacter pylori (H. pylori) is a serious pathogen, its origin is unknown. Poultry (Chicken, Turkey, Quebec, Goose, and Ostrich) are consumed as a regular protein source by a large number of people across the world; therefore, sanitary ways of delivering poultry for food are important for global health. As a result, we looked at the distribution of the pathogenicity cagA, vacA, babA2, oipA, and iceA in H. pylori isolates in poultry meat, as well as their antimicrobial resistance. Wilkins Chalgren anaerobic bacterial medium was used to cultivate 320 raw poultry specimens. Disk diffusion and Multiplex-PCR were used to investigate antimicrobial resistance and genotyping patterns, separately. H. pylori was found in 20 of 320 (6.25%) raw poultry samples. The highest incidence of H. pylori was found in chicken raw meat (15%), whereas the fewest was found in Goose and Quebec (0.00%). Resistance to ampicillin (85%), tetracycline (85%), and amoxicillin (75%) were greatest in H. pylori isolates. The percentage of H. pylori isolates with a MAR value of more than 0.2 was 17/20 (85%). The most prevalent genotypes discovered were VacA s1a (75%), m1a (75%), s2 (70%) and m2 (65%), and cagA (60%). The most typically discovered genotype patterns were s1am1a (45%), s2m1a (45%), and s2m2 (30%). BabA2, OipA + , and OipA− genotypes were found in 40%, 30%, and 30% of the population. In summary, the poultry flesh was polluted by H. pylori, with the babA2, vacA, and cagA genotypes being more prevalent. The simultaneous occurrence of vacA, cagA, iceA, oipA, and babA2 genotypes in antibiotic-resistant H. pylori bacteria implies a serious public health concern about raw poultry eating. In the future, researchers should look into H. pylori's resistance to multiple antibacterial drugs in Iran. Nature Publishing Group UK 2023-07-10 /pmc/articles/PMC10333334/ /pubmed/37430109 http://dx.doi.org/10.1038/s41598-023-38374-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Piri-Gharaghie, Tohid
Ghajari, Ghazal
Tolou-Shikhzadeh-Yazdi, Shakiba
Aghassizadeh-Sherbaf, Mona
Khorsand-Dehkordi, Sahar
Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
title Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
title_full Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
title_fullStr Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
title_full_unstemmed Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
title_short Helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
title_sort helicobacter pylori strains isolated from raw poultry meat: frequency and molecular characteristics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333334/
https://www.ncbi.nlm.nih.gov/pubmed/37430109
http://dx.doi.org/10.1038/s41598-023-38374-5
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