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Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato

Xanthomonas vesicatoria is one of the causal agents of bacterial spot, a disease that seriously affects the production of tomato (Solanum lycopersicum) and pepper (Capsicum annum) worldwide. In Argentina, bacterial spot is found in all tomato producing areas, with X. vesicatoria being one of the mai...

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Autores principales: Bianco, María Isabel, Ponso, María Agustina, Garita-Cambronero, Jerson, Conforte, Valeria Paola, Galván, Tadeo E., Dunger, Germán, Morales, Gustavo M., Vojnov, Adrián Alberto, Romero, Ana María, Cubero, Jaime, Yaryura, Pablo Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333488/
https://www.ncbi.nlm.nih.gov/pubmed/37440880
http://dx.doi.org/10.3389/fmicb.2023.1185368
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author Bianco, María Isabel
Ponso, María Agustina
Garita-Cambronero, Jerson
Conforte, Valeria Paola
Galván, Tadeo E.
Dunger, Germán
Morales, Gustavo M.
Vojnov, Adrián Alberto
Romero, Ana María
Cubero, Jaime
Yaryura, Pablo Marcelo
author_facet Bianco, María Isabel
Ponso, María Agustina
Garita-Cambronero, Jerson
Conforte, Valeria Paola
Galván, Tadeo E.
Dunger, Germán
Morales, Gustavo M.
Vojnov, Adrián Alberto
Romero, Ana María
Cubero, Jaime
Yaryura, Pablo Marcelo
author_sort Bianco, María Isabel
collection PubMed
description Xanthomonas vesicatoria is one of the causal agents of bacterial spot, a disease that seriously affects the production of tomato (Solanum lycopersicum) and pepper (Capsicum annum) worldwide. In Argentina, bacterial spot is found in all tomato producing areas, with X. vesicatoria being one of the main species detected in the fields. Previously, we isolated three X. vesicatoria strains BNM 208, BNM 214, and BNM 216 from tomato plants with bacterial spot, and found they differed in their ability to form biofilm and in their degree of aggressiveness. Here, the likely causes of those differences were explored through genotypic and phenotypic studies. The genomes of the three strains were sequenced and assembled, and then compared with each other and also with 12 other publicly available X. vesicatoria genomes. Phenotypic characteristics (mainly linked to biofilm formation and virulence) were studied in vitro. Our results show that the differences observed earlier between BNM 208, BNM 214, and BNM 216 may be related to the structural characteristics of the xanthan gum produced by each strain, their repertoire of type III effectors (T3Es), the presence of certain genes associated with c-di-GMP metabolism and type IV pili (T4P). These findings on the pathogenicity mechanisms of X. vesicatoria could be useful for developing bacterial spot control strategies aimed at interfering with the infection processes.
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spelling pubmed-103334882023-07-12 Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato Bianco, María Isabel Ponso, María Agustina Garita-Cambronero, Jerson Conforte, Valeria Paola Galván, Tadeo E. Dunger, Germán Morales, Gustavo M. Vojnov, Adrián Alberto Romero, Ana María Cubero, Jaime Yaryura, Pablo Marcelo Front Microbiol Microbiology Xanthomonas vesicatoria is one of the causal agents of bacterial spot, a disease that seriously affects the production of tomato (Solanum lycopersicum) and pepper (Capsicum annum) worldwide. In Argentina, bacterial spot is found in all tomato producing areas, with X. vesicatoria being one of the main species detected in the fields. Previously, we isolated three X. vesicatoria strains BNM 208, BNM 214, and BNM 216 from tomato plants with bacterial spot, and found they differed in their ability to form biofilm and in their degree of aggressiveness. Here, the likely causes of those differences were explored through genotypic and phenotypic studies. The genomes of the three strains were sequenced and assembled, and then compared with each other and also with 12 other publicly available X. vesicatoria genomes. Phenotypic characteristics (mainly linked to biofilm formation and virulence) were studied in vitro. Our results show that the differences observed earlier between BNM 208, BNM 214, and BNM 216 may be related to the structural characteristics of the xanthan gum produced by each strain, their repertoire of type III effectors (T3Es), the presence of certain genes associated with c-di-GMP metabolism and type IV pili (T4P). These findings on the pathogenicity mechanisms of X. vesicatoria could be useful for developing bacterial spot control strategies aimed at interfering with the infection processes. Frontiers Media S.A. 2023-06-27 /pmc/articles/PMC10333488/ /pubmed/37440880 http://dx.doi.org/10.3389/fmicb.2023.1185368 Text en Copyright © 2023 Bianco, Ponso, Garita-Cambronero, Conforte, Galván, Dunger, Morales, Vojnov, Romero, Cubero and Yaryura. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bianco, María Isabel
Ponso, María Agustina
Garita-Cambronero, Jerson
Conforte, Valeria Paola
Galván, Tadeo E.
Dunger, Germán
Morales, Gustavo M.
Vojnov, Adrián Alberto
Romero, Ana María
Cubero, Jaime
Yaryura, Pablo Marcelo
Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
title Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
title_full Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
title_fullStr Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
title_full_unstemmed Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
title_short Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
title_sort genomic and phenotypic insight into xanthomonas vesicatoria strains with different aggressiveness on tomato
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333488/
https://www.ncbi.nlm.nih.gov/pubmed/37440880
http://dx.doi.org/10.3389/fmicb.2023.1185368
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