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Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability
Nanodiamonds are increasingly popular in biomedical applications, including optical labelling, drug delivery and nanoscale sensing. Potential new applications are in studying infertility or labelling sperm cells. However, for these applications, it is necessary that nanodiamonds are inert and do not...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333662/ https://www.ncbi.nlm.nih.gov/pubmed/37441134 http://dx.doi.org/10.1016/j.mtbio.2023.100629 |
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author | San-Martin, Claudia Reyes Zhang, Yue Hamoh, Thamir Berendse, Lotte Klijn, Carline Li, Runrun Sigaeva, Alina Kawałko, Jakub Li, Hui Ting Tehrani, Jian Mzyk, Aldona Schirhagl, Romana |
author_facet | San-Martin, Claudia Reyes Zhang, Yue Hamoh, Thamir Berendse, Lotte Klijn, Carline Li, Runrun Sigaeva, Alina Kawałko, Jakub Li, Hui Ting Tehrani, Jian Mzyk, Aldona Schirhagl, Romana |
author_sort | San-Martin, Claudia Reyes |
collection | PubMed |
description | Nanodiamonds are increasingly popular in biomedical applications, including optical labelling, drug delivery and nanoscale sensing. Potential new applications are in studying infertility or labelling sperm cells. However, for these applications, it is necessary that nanodiamonds are inert and do not alter sperm properties. In this article, we assessed the biocompatibility of nanodiamonds in detail. We investigated different sizes and concentrations of nanodiamonds and sperm preparation methods. We evaluated if the metabolic activity, membrane integrity, morphology and formation of reactive oxygen species were altered. These parameters were tested for sperm cells in their uncapacitated and capacitated states. Unfortunately, FNDs are not universally biocompatible. Generally, cells in the capacitated state are more prone to stress. Additionally, larger particles and lower concentrations are tolerated better than smaller and higher concentrated particles. |
format | Online Article Text |
id | pubmed-10333662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103336622023-07-12 Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability San-Martin, Claudia Reyes Zhang, Yue Hamoh, Thamir Berendse, Lotte Klijn, Carline Li, Runrun Sigaeva, Alina Kawałko, Jakub Li, Hui Ting Tehrani, Jian Mzyk, Aldona Schirhagl, Romana Mater Today Bio Full Length Article Nanodiamonds are increasingly popular in biomedical applications, including optical labelling, drug delivery and nanoscale sensing. Potential new applications are in studying infertility or labelling sperm cells. However, for these applications, it is necessary that nanodiamonds are inert and do not alter sperm properties. In this article, we assessed the biocompatibility of nanodiamonds in detail. We investigated different sizes and concentrations of nanodiamonds and sperm preparation methods. We evaluated if the metabolic activity, membrane integrity, morphology and formation of reactive oxygen species were altered. These parameters were tested for sperm cells in their uncapacitated and capacitated states. Unfortunately, FNDs are not universally biocompatible. Generally, cells in the capacitated state are more prone to stress. Additionally, larger particles and lower concentrations are tolerated better than smaller and higher concentrated particles. Elsevier 2023-04-10 /pmc/articles/PMC10333662/ /pubmed/37441134 http://dx.doi.org/10.1016/j.mtbio.2023.100629 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Full Length Article San-Martin, Claudia Reyes Zhang, Yue Hamoh, Thamir Berendse, Lotte Klijn, Carline Li, Runrun Sigaeva, Alina Kawałko, Jakub Li, Hui Ting Tehrani, Jian Mzyk, Aldona Schirhagl, Romana Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability |
title | Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability |
title_full | Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability |
title_fullStr | Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability |
title_full_unstemmed | Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability |
title_short | Fluorescent nanodiamond labels: Size and concentration matters for sperm cell viability |
title_sort | fluorescent nanodiamond labels: size and concentration matters for sperm cell viability |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333662/ https://www.ncbi.nlm.nih.gov/pubmed/37441134 http://dx.doi.org/10.1016/j.mtbio.2023.100629 |
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