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Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics

Burn wound infection is the leading cause of mortality among burn wound patients. One of the most commonly isolated bacterial burn wound pathogens is Pseudomonas aeruginosa , a notorious nosocomial multidrug-resistant pathogen. As a consequence of its recalcitrance to frontline antibiotic therapy, t...

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Autores principales: Maslova, Evgenia, Osman, Shanga, McCarthy, Ronan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333784/
https://www.ncbi.nlm.nih.gov/pubmed/37350463
http://dx.doi.org/10.1099/mic.0.001350
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author Maslova, Evgenia
Osman, Shanga
McCarthy, Ronan R.
author_facet Maslova, Evgenia
Osman, Shanga
McCarthy, Ronan R.
author_sort Maslova, Evgenia
collection PubMed
description Burn wound infection is the leading cause of mortality among burn wound patients. One of the most commonly isolated bacterial burn wound pathogens is Pseudomonas aeruginosa , a notorious nosocomial multidrug-resistant pathogen. As a consequence of its recalcitrance to frontline antibiotic therapy, there is an urgent need to develop alternative treatment avenues to tackle this pathogen. One potential alternative infection prevention measure is to seed the wound bed with probiotic bacteria. Several species of Lactobacillus, a common commensal bacterium, have been previously reported to display growth inhibition activity against wound pathogens. Various species of this genus have also been shown to augment the wound healing process, which makes it a promising potential therapeutic agent. Due to the complexity of the burn wound trauma and burn wound infection, an in vivo model is required for the development of novel therapeutics. There are multiple in vivo models that are currently available, the most common among them being the murine model. However, mammalian burn wound infection models are logistically challenging, do not lend themselves to screening approaches and come with significant concerns around ethics and animal welfare. Recently, an invertebrate burn wound and infection model using G. mellonella has been established. This model addresses several of the challenges of more advanced animal models, such as affordability, maintenance and reduced ethical concerns. This study validates the capacity of this model to screen for potential wound probiotics by demonstrating that a variety of Lactobacillus spp. can limit P. aeruginosa burn wound infection and improve survival.
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spelling pubmed-103337842023-07-12 Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics Maslova, Evgenia Osman, Shanga McCarthy, Ronan R. Microbiology (Reading) Microbial Virulence and Pathogenesis Burn wound infection is the leading cause of mortality among burn wound patients. One of the most commonly isolated bacterial burn wound pathogens is Pseudomonas aeruginosa , a notorious nosocomial multidrug-resistant pathogen. As a consequence of its recalcitrance to frontline antibiotic therapy, there is an urgent need to develop alternative treatment avenues to tackle this pathogen. One potential alternative infection prevention measure is to seed the wound bed with probiotic bacteria. Several species of Lactobacillus, a common commensal bacterium, have been previously reported to display growth inhibition activity against wound pathogens. Various species of this genus have also been shown to augment the wound healing process, which makes it a promising potential therapeutic agent. Due to the complexity of the burn wound trauma and burn wound infection, an in vivo model is required for the development of novel therapeutics. There are multiple in vivo models that are currently available, the most common among them being the murine model. However, mammalian burn wound infection models are logistically challenging, do not lend themselves to screening approaches and come with significant concerns around ethics and animal welfare. Recently, an invertebrate burn wound and infection model using G. mellonella has been established. This model addresses several of the challenges of more advanced animal models, such as affordability, maintenance and reduced ethical concerns. This study validates the capacity of this model to screen for potential wound probiotics by demonstrating that a variety of Lactobacillus spp. can limit P. aeruginosa burn wound infection and improve survival. Microbiology Society 2023-06-22 /pmc/articles/PMC10333784/ /pubmed/37350463 http://dx.doi.org/10.1099/mic.0.001350 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Microbial Virulence and Pathogenesis
Maslova, Evgenia
Osman, Shanga
McCarthy, Ronan R.
Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
title Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
title_full Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
title_fullStr Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
title_full_unstemmed Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
title_short Using the Galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
title_sort using the galleria mellonella burn wound and infection model to identify and characterize potential wound probiotics
topic Microbial Virulence and Pathogenesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10333784/
https://www.ncbi.nlm.nih.gov/pubmed/37350463
http://dx.doi.org/10.1099/mic.0.001350
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