Cargando…

LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling

For the digestive system, there exists one common malignant tumor, known as gastric cancer. It is the third most prevalent type of tumor among different tumors worldwide. It has been reported that long noncoding RNAs (lncRNAs), participate in various biological processes of gastric cancer. However,...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yiming, Zheng, Wanqiong, Zhang, Liang, Gu, Yechun, Zhu, Lihe, Huang, Yingpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10334307/
https://www.ncbi.nlm.nih.gov/pubmed/37340903
http://dx.doi.org/10.4081/ejh.2023.3667
_version_ 1785070830104870912
author Zhang, Yiming
Zheng, Wanqiong
Zhang, Liang
Gu, Yechun
Zhu, Lihe
Huang, Yingpeng
author_facet Zhang, Yiming
Zheng, Wanqiong
Zhang, Liang
Gu, Yechun
Zhu, Lihe
Huang, Yingpeng
author_sort Zhang, Yiming
collection PubMed
description For the digestive system, there exists one common malignant tumor, known as gastric cancer. It is the third most prevalent type of tumor among different tumors worldwide. It has been reported that long noncoding RNAs (lncRNAs), participate in various biological processes of gastric cancer. However, there are still many lncRNAs with unknown functions, and we discovered a novel lncRNA designated as FBXO18-AS. Whether lncRNA FBXO18-AS participates in gastric cancer progression is still unknown. Bioinformatic analysis, immunohistochemistry, Western blotting, and qPCR were carried out to explore FBXO18-AS and TGF-β1 expression. In addition, EdU, MTS, migration and transwell assays were performed to investigate the invasion, proliferation and migration of gastric cancer in vitro. We first discovered that FBXO18-AS expression was upregulated in gastric cancer and linked to poorer outcomes among patients with gastric cancer. Then, we confirmed that FBXO18-AS promoted the proliferation, invasion, migration, and an EMT-like process in gastric cancer in vivo and in vitro. Mechanistically, FBXO18-AS was found to be involved in the progression of gastric cancer by modulating TGF-β1/Smad signaling. Therefore, it might offer a possible biomarker for gastric cancer diagnosis and an effective strategy for clinical treatment.
format Online
Article
Text
id pubmed-10334307
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher PAGEPress Publications, Pavia, Italy
record_format MEDLINE/PubMed
spelling pubmed-103343072023-07-12 LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling Zhang, Yiming Zheng, Wanqiong Zhang, Liang Gu, Yechun Zhu, Lihe Huang, Yingpeng Eur J Histochem Article For the digestive system, there exists one common malignant tumor, known as gastric cancer. It is the third most prevalent type of tumor among different tumors worldwide. It has been reported that long noncoding RNAs (lncRNAs), participate in various biological processes of gastric cancer. However, there are still many lncRNAs with unknown functions, and we discovered a novel lncRNA designated as FBXO18-AS. Whether lncRNA FBXO18-AS participates in gastric cancer progression is still unknown. Bioinformatic analysis, immunohistochemistry, Western blotting, and qPCR were carried out to explore FBXO18-AS and TGF-β1 expression. In addition, EdU, MTS, migration and transwell assays were performed to investigate the invasion, proliferation and migration of gastric cancer in vitro. We first discovered that FBXO18-AS expression was upregulated in gastric cancer and linked to poorer outcomes among patients with gastric cancer. Then, we confirmed that FBXO18-AS promoted the proliferation, invasion, migration, and an EMT-like process in gastric cancer in vivo and in vitro. Mechanistically, FBXO18-AS was found to be involved in the progression of gastric cancer by modulating TGF-β1/Smad signaling. Therefore, it might offer a possible biomarker for gastric cancer diagnosis and an effective strategy for clinical treatment. PAGEPress Publications, Pavia, Italy 2023-06-20 /pmc/articles/PMC10334307/ /pubmed/37340903 http://dx.doi.org/10.4081/ejh.2023.3667 Text en ©Copyright: the Author(s), https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Zhang, Yiming
Zheng, Wanqiong
Zhang, Liang
Gu, Yechun
Zhu, Lihe
Huang, Yingpeng
LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling
title LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling
title_full LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling
title_fullStr LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling
title_full_unstemmed LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling
title_short LncRNA FBXO18-AS promotes gastric cancer progression by TGF-β1/Smad signaling
title_sort lncrna fbxo18-as promotes gastric cancer progression by tgf-β1/smad signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10334307/
https://www.ncbi.nlm.nih.gov/pubmed/37340903
http://dx.doi.org/10.4081/ejh.2023.3667
work_keys_str_mv AT zhangyiming lncrnafbxo18aspromotesgastriccancerprogressionbytgfb1smadsignaling
AT zhengwanqiong lncrnafbxo18aspromotesgastriccancerprogressionbytgfb1smadsignaling
AT zhangliang lncrnafbxo18aspromotesgastriccancerprogressionbytgfb1smadsignaling
AT guyechun lncrnafbxo18aspromotesgastriccancerprogressionbytgfb1smadsignaling
AT zhulihe lncrnafbxo18aspromotesgastriccancerprogressionbytgfb1smadsignaling
AT huangyingpeng lncrnafbxo18aspromotesgastriccancerprogressionbytgfb1smadsignaling