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The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds
Persistence in the soil is a function of seed physiology, particularly non-germination and inherent lifespan. However, for seeds with mechanical dormancy, non-germination is also a function of the composition and activity of the soil microbiota. We attempted to screen out microorganisms in the soil...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335401/ https://www.ncbi.nlm.nih.gov/pubmed/37441178 http://dx.doi.org/10.3389/fpls.2023.1187614 |
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author | Wu, Yu Sun, Xiao-Rui Pritchard, Hugh W. Shen, Yong-Bao Wu, Xiao-Qin Peng, Chen-Yin |
author_facet | Wu, Yu Sun, Xiao-Rui Pritchard, Hugh W. Shen, Yong-Bao Wu, Xiao-Qin Peng, Chen-Yin |
author_sort | Wu, Yu |
collection | PubMed |
description | Persistence in the soil is a function of seed physiology, particularly non-germination and inherent lifespan. However, for seeds with mechanical dormancy, non-germination is also a function of the composition and activity of the soil microbiota. We attempted to screen out microorganisms in the soil that can specifically and rapidly decompose the hard fruit pericarps of Tilia miqueliana Maxim., a unique native tree species in China. Using the classical replica plating method, more than 100 different culturable microorganisms that could rapidly erode the pericarp were collected from the surface of pericarps under different culture conditions. At the same time, we successfully extended the concept of metagenomics and applied it to the identification of mixed artificial cultures. The decomposition process of the pericarps in soil was also simulated artificially. The physical and chemical data suggested a potential mechanism of microbial scarification and cracking in pericarp, whilst the embryos inside the eroded fruits retained good viability. Our discoveries could pave the way for the removal of physical and mechanical obstacles that prevent hard coat seeds from germinating. We anticipate that the use of this technology will improve the germination of other hard coat seeds. More research is needed to investigate the impacts on other seeds. The findings of this research can inform the design of experiments on the seed ecology of persistence. |
format | Online Article Text |
id | pubmed-10335401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103354012023-07-12 The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds Wu, Yu Sun, Xiao-Rui Pritchard, Hugh W. Shen, Yong-Bao Wu, Xiao-Qin Peng, Chen-Yin Front Plant Sci Plant Science Persistence in the soil is a function of seed physiology, particularly non-germination and inherent lifespan. However, for seeds with mechanical dormancy, non-germination is also a function of the composition and activity of the soil microbiota. We attempted to screen out microorganisms in the soil that can specifically and rapidly decompose the hard fruit pericarps of Tilia miqueliana Maxim., a unique native tree species in China. Using the classical replica plating method, more than 100 different culturable microorganisms that could rapidly erode the pericarp were collected from the surface of pericarps under different culture conditions. At the same time, we successfully extended the concept of metagenomics and applied it to the identification of mixed artificial cultures. The decomposition process of the pericarps in soil was also simulated artificially. The physical and chemical data suggested a potential mechanism of microbial scarification and cracking in pericarp, whilst the embryos inside the eroded fruits retained good viability. Our discoveries could pave the way for the removal of physical and mechanical obstacles that prevent hard coat seeds from germinating. We anticipate that the use of this technology will improve the germination of other hard coat seeds. More research is needed to investigate the impacts on other seeds. The findings of this research can inform the design of experiments on the seed ecology of persistence. Frontiers Media S.A. 2023-06-27 /pmc/articles/PMC10335401/ /pubmed/37441178 http://dx.doi.org/10.3389/fpls.2023.1187614 Text en Copyright © 2023 Wu, Sun, Pritchard, Shen, Wu and Peng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wu, Yu Sun, Xiao-Rui Pritchard, Hugh W. Shen, Yong-Bao Wu, Xiao-Qin Peng, Chen-Yin The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
title | The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
title_full | The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
title_fullStr | The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
title_full_unstemmed | The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
title_short | The metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
title_sort | metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335401/ https://www.ncbi.nlm.nih.gov/pubmed/37441178 http://dx.doi.org/10.3389/fpls.2023.1187614 |
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