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A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development
To respond to the world around them, animals rely on the input of a network of sensory organs distributed throughout the body. Distinct classes of sensory organs are specialized for the detection of specific stimuli such as strain, pressure, or taste. The features that underlie this specialization r...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335707/ https://www.ncbi.nlm.nih.gov/pubmed/37379332 http://dx.doi.org/10.1371/journal.pbio.3002148 |
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author | Hopkins, Ben R. Barmina, Olga Kopp, Artyom |
author_facet | Hopkins, Ben R. Barmina, Olga Kopp, Artyom |
author_sort | Hopkins, Ben R. |
collection | PubMed |
description | To respond to the world around them, animals rely on the input of a network of sensory organs distributed throughout the body. Distinct classes of sensory organs are specialized for the detection of specific stimuli such as strain, pressure, or taste. The features that underlie this specialization relate both to the neurons that innervate sensory organs and the accessory cells they comprise. To understand the genetic basis of this diversity of cell types, both within and between sensory organs, we performed single-cell RNA sequencing on the first tarsal segment of the male Drosophila melanogaster foreleg during pupal development. This tissue displays a wide variety of functionally and structurally distinct sensory organs, including campaniform sensilla, mechanosensory bristles, and chemosensory taste bristles, as well as the sex comb, a recently evolved male-specific structure. In this study, we characterize the cellular landscape in which the sensory organs reside, identify a novel cell type that contributes to the construction of the neural lamella, and resolve the transcriptomic differences among support cells within and between sensory organs. We identify the genes that distinguish between mechanosensory and chemosensory neurons, resolve a combinatorial transcription factor code that defines 4 distinct classes of gustatory neurons and several types of mechanosensory neurons, and match the expression of sensory receptor genes to specific neuron classes. Collectively, our work identifies core genetic features of a variety of sensory organs and provides a rich, annotated resource for studying their development and function. |
format | Online Article Text |
id | pubmed-10335707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-103357072023-07-12 A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development Hopkins, Ben R. Barmina, Olga Kopp, Artyom PLoS Biol Methods and Resources To respond to the world around them, animals rely on the input of a network of sensory organs distributed throughout the body. Distinct classes of sensory organs are specialized for the detection of specific stimuli such as strain, pressure, or taste. The features that underlie this specialization relate both to the neurons that innervate sensory organs and the accessory cells they comprise. To understand the genetic basis of this diversity of cell types, both within and between sensory organs, we performed single-cell RNA sequencing on the first tarsal segment of the male Drosophila melanogaster foreleg during pupal development. This tissue displays a wide variety of functionally and structurally distinct sensory organs, including campaniform sensilla, mechanosensory bristles, and chemosensory taste bristles, as well as the sex comb, a recently evolved male-specific structure. In this study, we characterize the cellular landscape in which the sensory organs reside, identify a novel cell type that contributes to the construction of the neural lamella, and resolve the transcriptomic differences among support cells within and between sensory organs. We identify the genes that distinguish between mechanosensory and chemosensory neurons, resolve a combinatorial transcription factor code that defines 4 distinct classes of gustatory neurons and several types of mechanosensory neurons, and match the expression of sensory receptor genes to specific neuron classes. Collectively, our work identifies core genetic features of a variety of sensory organs and provides a rich, annotated resource for studying their development and function. Public Library of Science 2023-06-28 /pmc/articles/PMC10335707/ /pubmed/37379332 http://dx.doi.org/10.1371/journal.pbio.3002148 Text en © 2023 Hopkins et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Methods and Resources Hopkins, Ben R. Barmina, Olga Kopp, Artyom A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development |
title | A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development |
title_full | A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development |
title_fullStr | A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development |
title_full_unstemmed | A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development |
title_short | A single-cell atlas of the sexually dimorphic Drosophila foreleg and its sensory organs during development |
title_sort | single-cell atlas of the sexually dimorphic drosophila foreleg and its sensory organs during development |
topic | Methods and Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10335707/ https://www.ncbi.nlm.nih.gov/pubmed/37379332 http://dx.doi.org/10.1371/journal.pbio.3002148 |
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