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An investigation of genetic diversity in three Dezhou donkey original breeding farms

Dezhou donkey is one of the excellent large donkey breeds in China. In our study, eight microsatellite markers were used to genotype from each of three populations of Chinese Dezhou donkeys: 67 individuals in Liaocheng (pop1), 103 individuals in Binzhou 1 (pop2), and 102 individuals in Binzhou 2 (po...

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Autores principales: Wang, Tianqi, Liu, Ziwen, Shi, Xiaoyuan, Zhang, Zhenwei, Li, Yuhua, Huang, Bingjian, Ren, Wei, Wang, Xinrui, Wang, Changfa, Chai, Wenqiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336002/
https://www.ncbi.nlm.nih.gov/pubmed/37433834
http://dx.doi.org/10.1038/s41598-023-38219-1
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author Wang, Tianqi
Liu, Ziwen
Shi, Xiaoyuan
Zhang, Zhenwei
Li, Yuhua
Huang, Bingjian
Ren, Wei
Wang, Xinrui
Wang, Changfa
Chai, Wenqiong
author_facet Wang, Tianqi
Liu, Ziwen
Shi, Xiaoyuan
Zhang, Zhenwei
Li, Yuhua
Huang, Bingjian
Ren, Wei
Wang, Xinrui
Wang, Changfa
Chai, Wenqiong
author_sort Wang, Tianqi
collection PubMed
description Dezhou donkey is one of the excellent large donkey breeds in China. In our study, eight microsatellite markers were used to genotype from each of three populations of Chinese Dezhou donkeys: 67 individuals in Liaocheng (pop1), 103 individuals in Binzhou 1 (pop2), and 102 individuals in Binzhou 2 (pop3), in order to study the genetic diversity of these populations. A total of 213 alleles were detected, and the PIC results showed that eight loci were all highly polymorphic. The means of Ho and He of pop2 were the highest, which were 0.646 and 0.717, respectively. The PCoA analysis demonstrated that the samples from three conservation farms were mixed together. The phylogenetic tree showed that pop 2 and pop 3 were closely related. The phylogenetic tree results clustered that 272 donkeys were divided into six groups. AMOVA analysis showed that the genetic variation was mainly concentrated within the population and the genetic differentiation among the populations was low. Fst values between populations also indicated that genetic differentiation between populations was too small to be considered. This indicated a low probability of inbreeding within the population. And this also showed that the conservation and breeding of Dezhou donkeys in recent years have achieved excellent results. The investigation of genetic diversity in three Dezhou donkey original breeding farms can provide reference data for the selection and breeding of excellent breeds of Dezhou donkey.
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spelling pubmed-103360022023-07-13 An investigation of genetic diversity in three Dezhou donkey original breeding farms Wang, Tianqi Liu, Ziwen Shi, Xiaoyuan Zhang, Zhenwei Li, Yuhua Huang, Bingjian Ren, Wei Wang, Xinrui Wang, Changfa Chai, Wenqiong Sci Rep Article Dezhou donkey is one of the excellent large donkey breeds in China. In our study, eight microsatellite markers were used to genotype from each of three populations of Chinese Dezhou donkeys: 67 individuals in Liaocheng (pop1), 103 individuals in Binzhou 1 (pop2), and 102 individuals in Binzhou 2 (pop3), in order to study the genetic diversity of these populations. A total of 213 alleles were detected, and the PIC results showed that eight loci were all highly polymorphic. The means of Ho and He of pop2 were the highest, which were 0.646 and 0.717, respectively. The PCoA analysis demonstrated that the samples from three conservation farms were mixed together. The phylogenetic tree showed that pop 2 and pop 3 were closely related. The phylogenetic tree results clustered that 272 donkeys were divided into six groups. AMOVA analysis showed that the genetic variation was mainly concentrated within the population and the genetic differentiation among the populations was low. Fst values between populations also indicated that genetic differentiation between populations was too small to be considered. This indicated a low probability of inbreeding within the population. And this also showed that the conservation and breeding of Dezhou donkeys in recent years have achieved excellent results. The investigation of genetic diversity in three Dezhou donkey original breeding farms can provide reference data for the selection and breeding of excellent breeds of Dezhou donkey. Nature Publishing Group UK 2023-07-11 /pmc/articles/PMC10336002/ /pubmed/37433834 http://dx.doi.org/10.1038/s41598-023-38219-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Tianqi
Liu, Ziwen
Shi, Xiaoyuan
Zhang, Zhenwei
Li, Yuhua
Huang, Bingjian
Ren, Wei
Wang, Xinrui
Wang, Changfa
Chai, Wenqiong
An investigation of genetic diversity in three Dezhou donkey original breeding farms
title An investigation of genetic diversity in three Dezhou donkey original breeding farms
title_full An investigation of genetic diversity in three Dezhou donkey original breeding farms
title_fullStr An investigation of genetic diversity in three Dezhou donkey original breeding farms
title_full_unstemmed An investigation of genetic diversity in three Dezhou donkey original breeding farms
title_short An investigation of genetic diversity in three Dezhou donkey original breeding farms
title_sort investigation of genetic diversity in three dezhou donkey original breeding farms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336002/
https://www.ncbi.nlm.nih.gov/pubmed/37433834
http://dx.doi.org/10.1038/s41598-023-38219-1
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