A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

The methylotrophic budding yeast Pichia pastoris has been utilized to the production of a variety of heterologous recombinant proteins owing to the strong inducible alcohol oxidase promoter (pAOX1). However, it is difficult to use P. pastoris as the chassis cell factory for high-valuable metabolite...

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Detalles Bibliográficos
Autores principales: Wang, Xiang, Li, Yi, Jin, Zhehao, Liu, Xiangjian, Gao, Xiang, Guo, Shuyuan, Yu, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2023
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336193/
https://www.ncbi.nlm.nih.gov/pubmed/37448527
http://dx.doi.org/10.1016/j.synbio.2023.06.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336193/
https://www.ncbi.nlm.nih.gov/pubmed/37448527
http://dx.doi.org/10.1016/j.synbio.2023.06.003

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