Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms

Understanding the mechanisms of action of adjuvants at the tissue level is crucial to the development of more potent and safer versions for human use. Comparative tissue proteomics presents a novel tool to study their unique action mechanisms. Here, we present a protocol for preparing murine tissue...

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Detalles Bibliográficos
Autores principales: Li, Yibo, Chen, Xinyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336302/
https://www.ncbi.nlm.nih.gov/pubmed/37393612
http://dx.doi.org/10.1016/j.xpro.2023.102396
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author Li, Yibo
Chen, Xinyuan
author_facet Li, Yibo
Chen, Xinyuan
author_sort Li, Yibo
collection PubMed
description Understanding the mechanisms of action of adjuvants at the tissue level is crucial to the development of more potent and safer versions for human use. Comparative tissue proteomics presents a novel tool to study their unique action mechanisms. Here, we present a protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms. We describe steps for adjuvant treatment in live animals, tissue harvesting, and homogenization. We then detail protein extraction and digestion to prepare for liquid chromatography-tandem mass spectrometry analysis. For complete details on the use and execution of this protocol, please refer to Li et al.(1)
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spelling pubmed-103363022023-07-13 Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms Li, Yibo Chen, Xinyuan STAR Protoc Protocol Understanding the mechanisms of action of adjuvants at the tissue level is crucial to the development of more potent and safer versions for human use. Comparative tissue proteomics presents a novel tool to study their unique action mechanisms. Here, we present a protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms. We describe steps for adjuvant treatment in live animals, tissue harvesting, and homogenization. We then detail protein extraction and digestion to prepare for liquid chromatography-tandem mass spectrometry analysis. For complete details on the use and execution of this protocol, please refer to Li et al.(1) Elsevier 2023-07-01 /pmc/articles/PMC10336302/ /pubmed/37393612 http://dx.doi.org/10.1016/j.xpro.2023.102396 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Yibo
Chen, Xinyuan
Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
title Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
title_full Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
title_fullStr Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
title_full_unstemmed Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
title_short Protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
title_sort protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336302/
https://www.ncbi.nlm.nih.gov/pubmed/37393612
http://dx.doi.org/10.1016/j.xpro.2023.102396
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